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Volume 7, Issue 3(Suppl) J Biotechnol Biomater, an open access journal
ISSN: 2155-952X Euro Biotechnology 2017
September 25-27, 2017
17th EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Atrazine degradation by Arthrobacter sp. ZXY-2: Kinetics, pathway, gene expression response and genomic
characterization
Xinyue Zhao1,2, Jixian Yang1, Li Wang1 and Fang Ma 1
1Harbin Institute of Technology, China
2Delft University of Technology, Netherlands
Isolation of atrazine-degrading microorganisms with specific characteristics is significant for the bio-augmentation to deal with
atrazine wastewater. However, lacking the investigation of specific characteristics will hinder the further understanding of bio-
augmentation. A strain Arthrobacter sp. ZXY-2 with strong capacity of atrazine degradation has been isolated and suggested a
potential candidate for bio-augmentation. In this study, we identified the factors that might be relevant to the biodegradation capacity
of strain ZXY-2, and reveal how these factors might contribute to the future understanding of bio-augmentation. The growth pattern
of strain ZXY-2 followed Haldane-Andrew model with the inhibition constant (Ki) of 52.76 mg/L obtained, indicating that the
strain ZXY-2 offered a possibility of bio-augmenting wastewater with the concentration of atrazine below 52.76 mg/L. The Real-
time quantitative PCR (RT-qPCR) results showed a positive correlation between atrazine degradation and the expression levels of
functional genes (trzN, atzB and atzC), which provided a basis data that could help to distinguish the role strain ZXY-2 played in
the bio-augmentation. Moreover, the multiple copies of atzB gene, found via genome sequencing, might account for the highest
expression levels among three genes. Meanwhile, the multiple copies of atzB gene might also provide a compensation mechanism to
ensure the smooth work of strain ZXY-2 in future bio-augmentation.
Biography
Xinyue Zhao completed her Master’s degree in Environmental Microbiology at Harbin Institute of Technology (HIT) in July 2014. Then, she continued her PhD
research, majored in Environmental Science and Engineering as a visiting scholar-PhD student from September 2016 at Delft University of Technology. She has
published six papers during her PhD study.
Xinyue Zhao et al., J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-076