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Volume 7, Issue 3(Suppl) J Biotechnol Biomater, an open access journal
ISSN: 2155-952X Euro Biotechnology 2017
September 25-27, 2017
17th EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Characterizing constitutive promoters in yeast
Sabrina Schulze
University of Potsdam, Germany
Design and establishment of new biosynthetic pathways in yeast are important goals in synthetic biology. Therefore, promoters
with predictable and reproducible protein expression levels independent of the protein of interest are needed. Data presented here
show a library of constitutive promoters expressing two different fluorescent proteins in diverse conditions. We compare expression
levels for episomal and chromosomal location, different growth media and different growth times. GFP and RFP are quantified via
fluorescence spectroscopy and flow cytometry.
Biography
Sabrina Schulze completed her PhD in Medical Sciences at the University of Aberdeen (UK) followed by a Postdoctoral Associate position at the University of
Pittsburgh (USA). In 2017, she joined the group for synthetic biosystems at Potsdam University (Germany).
Sabrina Schulze, J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-076