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Volume 7, Issue 3(Suppl) J Biotechnol Biomater, an open access journal
ISSN: 2155-952X Euro Biotechnology 2017
September 25-27, 2017
17th EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Application of laccase during the attainment of PVA hydrogels reticulated with ferulic acid
Andreia de Araújo Morandim-Giannetti1, Victor Hugo Escobar1, Fernando dos Santos Ortega1, Octaviano Magalhães Junior2, Paulo Schor2 and Patrícia
Alessandra Bersanetti2
1Centro Universitário da FEI, Brazil
2Escola Paulista de Medicina, Brazil
Many researches have been aiming at obtaining new possible vitreous humor substitutes. Therefore, we performed studies
related to obtain hydrogels enzymatically cross-linked with ferulic acid. The hydrogels were obtained by varying PVA mass,
enzyme concentration and the mass of ferulic acid, to obtain the best condition to have a material with similar characteristics in
terms of density (1.0053 to 1.0089 g/mL), kinematic viscosity (greater than 4mm2/cm) and refractive index (between 1.3345 and
1.3348). The data was analysed using the Statistica 12.0 software, and it was possible to determine the best condition for obtaining
the material: mPVA=12.05% (m/m), laccase concentration=836 (μg/mL) and ferulic acid concentration=1.95 (mM). Hydrogels were
obtained under the best condition and on analyzing by DSC, confirmed the presence of crosslinks in the hydrogels and reinforced
the hypothesis of the presence of crosslinks due to the action of the Laccase enzyme. This was justified due to a reduction of the glass
transition temperature (69.99 and 74.49 ºC), melt temperature (216.48 and 220.26 ºC) and crystallization temperature (181.82 and
184.62 ºC), as well as the degree of crystallinity (29.18 and 29.74 %) for the hydrogel obtained with and without PVA, ferulic acid
and laccase, respectively. In this case, it is possibly attributed to the greater intensity of the hydrogen bonds between the PVA chains,
which makes it difficult to move and pack the chains into crystallites.
Biography
Andreia de Araújo Morandim Giannetti has completed her PhD and Postdoctoral studies from Paulista State University (UNESP). She is a teacher at FEI University
Center. She has published more than 18 papers in reputed journals and has been serving as a reviewer in several renowned journals.
Andreia de Araújo Morandim-Giannetti et al., J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-076