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Volume 7, Issue 3(Suppl) J Biotechnol Biomater, an open access journal
ISSN: 2155-952X Euro Biotechnology 2017
September 25-27, 2017
17th EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Free floating brain sections for immunofluorescence markers: A technical and scientific approach
Emmanuel Loeb
Patho-Logica, Scientific Park Ness Ziona, Israel
Free floating sections is regarded as a new histological method that can be used for immune fluorescence staining. This method is
clearly the best way to go for optimal Ab expression in the tissue. Furthermore, staining of thick sections can later on be used for a
confocal microscopical analysis. This presentation covers the technical work pattern of the method starting with the tissue preparation
and conservation, threw brain accurate dissection and staining. The method is very suitable for morphometry quantification of
histological data, here method of image analysis will be presented and the scientific value will be discussed. Furthermore, examples
are presented of projects that had combined the method such as stroke and Parkinson models in lab animals. Finally a discussion will
be presented were the advantages of the current method will be pointed compared to the classical immunohistochemistry methods.
Biography
Emmanuel Loeb is a graduate from School of Veterinary Medicine, Utrecht University, Netherlands and a qualified expert, Veterinary Pathologist with published
papers. He has 12 years of experience in Experimental Pathology and is constantly improving his skills through continuous profession development. In his work,
he takes part in annual professional meetings such as the ESVP and follows The Society of Toxicologic Pathology recommendations. He established new methods
in the laboratory such as “free floating sections” for immunofluorescence staining, and developed translation tools from pathological hallmarks to histological end
point. He is also teaching pathology at the Veterinary School of Koret (Hebrew University).
Emmanuel Loeb, J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-076