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.com
Volume 7, Issue 3(Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Euro Biotechnology 2017
September 25-27, 2017
17
th
EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
The effects of subculture methods on browning of callus derived from
Corylus
avellana L cotyledons
Sara Alsadat Rahpeyma
1
, Mona Raeispour Shirazi
1
, Sajad Rashidi Monfared
2
and
Jafar Zolala
1
1
Shahid Bahonar University, Iran
2
Tarbiat Modares University, Iran
H
azel has been reported as a taxol-producing species, which is a potent antimitotic drug employed in many different cancer
treatments, through bioprospection among angiosperms. Plant cell culture is considered as one of the most promising
approaches to provide a stable supply of taxol and related compounds, generally named taxanes. Callus browning is a major problem
of callus cultures derived from the cotyledon of
C. avellana
L. Brown callus results in decreasing regenerative ability, poor growth
and even death, especially through the first subculture. In this study, we investigated the effect of subculture methods on reduction of
callus browning. Two subculture methods were applied: consecutive subculture the cotyledons callus in the same solid medium for 4
weeks; subculture the cotyledons callus in the liquid medium with the same composition and then immobilized the cells on the solid
medium. The results indicated that subculture the callus in the liquid and then solid medium can practically reduce callus browning
and increase the viability of callus.
Biography
Sara Rahpeyma is an Assistant Professor of Plant Breeding Engineering in Department of Agricultural Biotechnology at Shahid Bahonar University of Kerman, Iran.
Her research programs have been on plant secondary metabolites and also, her graduate work focused on enhancing the production of paclitaxel in cell suspension
culture of
Corylus avellana
.
s.rahpeyma@uk.ac.irSara Alsadat Rahpeyma et al., J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-077