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Volume 7, Issue 3(Suppl)

J Biotechnol Biomater, an open access journal

ISSN: 2155-952X

Euro Biotechnology 2017

September 25-27, 2017

EURO BIOTECHNOLOGY CONGRESS

September 25-27, 2017 Berlin, Germany

Differential expression of anthocyanin biosynthesis genes and transcription factors determines coloration

patterns in gerbera flowers

Hyun Young Song, Aung Htay Naing

and

Chang Kil Kim

Kyungpook National University, Korea

iverse flower colors exist in different gerbera cultivars. To elucidate the different coloration patterns in two commercial cultivars

‘Nathasha and Rosalin’, expressions of anthocyanin biosynthesis genes and transcription factors associated with varying

anthocyanin contents during different developmental stages (S1 to S5) were investigated. In addition, role of different temperatures

in anthocyanin biosynthesis were also investigated by detecting anthocyanin content and gene expression levels. Accumulation of

anthocyanin in both cultivars started at S1 and reached a maximum at both of S2 and S3 or only S3 depending on the cultivars.

Enhancement of anthocyanin in cv. Nathasha was associated with up-regulation of

ANS

and

MYB10,

while

CHS1

and MYC were

likely to be responsible for this in cv. Rosalin. Low temperature (6 ºC) could enhance the anthocyanin contents than 22 ºC by stronger

up-regulation of

CHS1

and MYB10 in cv. Nathasha or of

CHS1

and MYC in cv. Rosalin, regardless of the flower stages. However, the

difference of contents between the two cultivars was found to be influenced by expression levels of all biosynthesis genes and TFs,

regardless of flower stages and temperature conditions. Hence, it was suggested that the expression patterns of biosynthesis genes

and TFs are involved in the differential regulation mechanisms of anthocyanin biosynthesis and coloration pattern between the two

cultivars, although further functional studies of the key genes still need to be explored.

Biography

Hyun Young Song is doing Master Degree at Kyungpook National University, South Korea.

Hyun Young Song et al., J Biotechnol Biomater 2017, 7:3(Suppl)

DOI: 10.4172/2155-952X-C1-077