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Volume 7, Issue 3(Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Euro Biotechnology 2017
September 25-27, 2017
17
th
EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Effects of plants growth regulators and carbon sources on
in vitro
shoot elongation, rooting, and plantlet
acclimatization of date palm (
Phoenix dactylifera
L.) cv. Mejhoul
Meziani Reda
1,2
, Mazri Mouaad Amine
1
and
Jaiti Fatima
2
1
Institut National de la Recherche Agronomique, Morocco
2
Facult des sciences et techniques, Errachidia, Morocco
D
ate palm (
Phoenix dactylifera
L.) is an agronomically, ecologically and socio-economically important fruit tree in many countries.
This species is mainly propagated by somatic embryogenesis. However, this technique may result in somaclonal variation within
regenerants. Recently, date palm micro-propagation through organogenesis has gained much interest since it allows to produce
true-to-type plantlets. Organogenesis is the technique by which adventitious buds are formed directly on the explant. It comprises
numerous steps: initiation of vegetative buds, bud multiplication, shoot elongation and rooting then plantlet acclimatization. In
previous works, we evaluated the effects of numerous factors on bud initiation and multiplication. Thus, the purpose of this study
was to evaluate the effects of different plant growth regulator combinations and carbon sources on shoot elongation, rooting and
plantlet acclimatization of date palm cv. Mejhoul. The results of this study showed that the combination of 1 mg/L NAA, 1 mg/L
BAP and 1 mg/L KIN resulted in the highest leaf length with an average of 19.2 cm. The use of KIN alone in the culture medium
resulted in leaf lengths ranging from 12 to 14 cm. Root formation was strongly stimulated using NAA alone or in combination with
IBA. Regarding leaf greening, the PGR-free medium gave the highest chlorophyll content with 6.78 CCI. After one month in the
glasshouse, the plantlet survival rate was higher within those that have been grown on PGR-free medium. On the other hand, the
carbon source (sucrose, mannitol, sorbitol or commercial granular sugar) showed a significant effect on shoot development and
plantlet acclimatization. The use of sucrose gave the best results
in vitro
and
ex vitro
, with an average shoot length of 13.6 cm, a high
chlorophyll content (10.04 CCI), and a high survival rate after acclimatization 80%. The use of commercial sugar as carbon source has
also given satisfactory results, with a survival rate of 70 %.
Biography
Meziani Reda has obtained his Engineer Degree from the National School of Agriculture of Meknes in 2009. He is currently a PhD student in University Moulay
Ismail, Faculty of Science and Technology of Errachidia. His research is focused on the micro-propagation of date palm. He has published many papers in reputed
journals and participated to many international congresses in numerous countries.
redameziani@yahoo.fr.
Meziani Reda et al., J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-077