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.com
Volume 7, Issue 3(Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Euro Biotechnology 2017
September 25-27, 2017
17
th
EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Overexpression of snapdragon
Delila (Del)
gene in tobacco enhances anthocyanin accumulation and abiotic
stress tolerance
Aung Htay Naing
and
Chang Kil Kim
Kyungpook National University, South Korea
R
osea1 (
Ros1
) and Del (
Delila
) co-expression controls anthocyanin accumulation in snapdragon flowers, while their
overexpression in tomato strongly induces anthocyanin accumulation. However, little data exist on how Del expression alone
influences anthocyanin accumulation. In tobacco (
Nicotiana tabacum ‘Xanthi’
), Del expression enhanced leaf and flower anthocyanin
production through regulating
NtCHS, NtCHI, NtF3H, NtDFR,
and
NtANS
transcript levels. Transgenic lines displayed different
anthocyanin colors (e.g., pale red: T
0
-P, red: T
0
-R, and strong red: T
0
-S), resulting from varying levels of biosynthetic gene transcripts.
Under salt stress, the T2 generation had higher total polyphenol content, radical (DPPH, ABTS) scavenging activities, antioxidant-
related gene expression, as well as overall greater salt and drought tolerance than wild type. We propose that Del overexpression
elevates transcript levels of anthocyanin biosynthetic and antioxidant-related genes, leading to enhanced anthocyanin production
and antioxidant activity. The resultant increase of anthocyanin and antioxidant activity improves abiotic stress tolerance.
Biography
Aung Htay Naing has completed his PhD from Kyungpook National University, South Korea. He has published more than 30 papers in SCI/E in journals and has
been serving as an Editorial Board Member of some plant science journals.
aunghtaynaing2005@gmail.comAung Htay Naing et al., J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-077