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.com
Volume 7, Issue 3(Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Euro Biotechnology 2017
September 25-27, 2017
17
th
EURO BIOTECHNOLOGY CONGRESS
September 25-27, 2017 Berlin, Germany
Determination of phytotron optimal condition for
in vitro
potato ontogenesis
Maia Kukhaleishvili, Ekaterine Bulauri, Tamar Chipashvili, Tamar Shamatava
and
Iveta Megrelishvili
Georgian Technical University, Georgia
T
hree type of combinations with light, temperature, humidity and photoperiod was made: 1. 22-23
o
C, 4000 lux, humidity
70%, 16h. 2. 24-25
o
C, 5000 lux, humidity 75%, 18h. 3. 26-27
o
C, 6000 lux, humidity 80 %, 20h. These were studied on
in vitro
cultivation of potato cultivars: Sebago, Russet Burbank, Katahdin and Carola for 21-24 days. All
in vitro
potato cultivars morphological
characterization was variable depending on the type of
in vitro
condition combination. It was revealed that all researched potato
varieties had maximum potential for
in vitro
propagation (Green leaves, rooting 90% and shoot formation 94%) on combination
of: 24-25
o
C, 5000 lux, humidity 75% and 18h after 17 days of cultivation. Plant development (Green leaves, rooting 87% and shoot
formation 92%) on the
in vitro
condition combination: 22-230C, 4000lux, humidity 70% and 16h was completed after 21 days. And
in vitro
shoot and root formation (light green leaves, rooting 89% and shoot formation 78%) on combination of: 26-27
o
C, 6000 lux,
humidity 80% and 20h was presented after 14 days of cultivation. Best combination (26-27
o
C, 6000 lux, humidity 80% and 20h) of
in
vitro
condition for all researched potato varieties
in vitro
cultivation was selected for their leave colors, rooting, and shoot formation.
Biography
Maia Kukhaleishvili has completed her PhD from ST. Andrew the First Called Georgian University of the Patriarchate of Georgia. She is the Director of Georgian
Technical University, Biotechnology Center- Scientific-Research Center. She has published more than 15 papers in reputed journals and has a vast experience in
Agriculture and Biotechnology sphere.
maia.kukh@gmail.comMaia Kukhaleishvili et al., J Biotechnol Biomater 2017, 7:3(Suppl)
DOI: 10.4172/2155-952X-C1-077