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conferenceseries.com
Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
The investigation of effects of commercial protease and Bacillus subtilis 168 E6-5 protease on felting and
dyeing behaviour of 100% wool fabric
Tuba Sevgi, Elif Demirkan, Dilek Kut and Meral Dogan
Uludag University, Turkey
In this study, a novel bacterial strain with high protease activity (210 U/ml) was isolated from soil, and then identified by its
morphological character and 16S rRNA sequence, and named Bacillus subtilis 168 E6-5. Bacillus protease enzyme and commercial
protease enzyme were applied to 100% raw wool fabric and bleached wool fabric. After dyeing with acid dyes, changes in the size
of the fabric and color yields were measured. Protease was purified by dialysis+lyophilization, and applied on dyed wool fabric and
felting shrinkage values were measured. Enzyme treated and dyed wool fabric possess 8%, however non-treated wool fabric has 11%
of felting shrinkage value just after dyeing step. After performing five repeated washing, the enzyme treated raw fabric has 12% and the
non-treated raw fabric has 15%. After pre-washing, bleaching and dyeing steps, the felting shrinkage value of the enzyme treated wool
fabric was 9%, while non-treated one was 11%. After the processes of pre-treatment, bleaching and dying the K/S value indicating
the colour yield of the fabric was measued. The K/S value of the wool fabric that was treated with enzyme before the processes of
pretreatment, bleaching and dying was 31.68, while the non-enzyme-treated wool fabric has 26.33. Enzyme application increased the
colour yield. This study suggests that the Bacillus protease enzyme shows better results in behaviours of felting and dying than the
commercial protease enzyme and applicable on wool fabrics. Therefore, this protease enzyme has potential in textile industry.
Biography
Tuba Sevgi has completed her MSc from Technical University of Kaiserslautern in Molecular Biotechnology and Systems Biology, Germany. Currently, she is doing
her PhD in the Department of Biology, Faculty of Arts and Sciences, Uludag University. She is a Research Assistant in the Department of Biology, Faculty of Arts
and Sciences, Uludag University.
Tuba Sevgi et al., J Biotechnol Biomater 2017, 7:1(Suppl)