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Volume 7, Issue 1 (Suppl)

J Biotechnol Biomater

ISSN: 2155-952X JBTBM, an open access journal

March 20-21, 2017 Rome, Italy

&

15

th

World Congress on

2

nd

International Conference on

Biotechnology And Biotech Industries Meet

Enzymology and Molecular Biology

Enzymology & Mol. Biology 2017

Biotechnology Congress 2017

March 20-21, 2017

How to tune recombinant protein production in

E. coli

for enhanced production of biopharmaceuticals?

Oliver Spadiut

TU Wien, Austria

S

trong induction of recombinant protein production in

E

.

coli

can lead to agglomeration of inactive product, inclusion bodies

(IBs), and also imposes a high metabolic burden which can result in cell death. We developed a feeding strategy using glucose

as primary carbon source, lactose as secondary carbon source and inducer to tune recombinant protein expression which leads to

higher yields of soluble and active product. We successfully applied this system for the production of several biopharmaceuticals.

This new feeding approach allows expression of complex products as soluble and active protein that usually results in insoluble and

inactive inclusion bodies. Cell viability and growth can be prolonged by this approach which leads to higher overall yields and thus

lower production costs. Thus, our strategy might make

E. coli

a more attractive host for the production of biopharmaceuticals in the

future. The audience will get to know a platform technology for the enhanced expression of biopharmaceuticals in

E. coli

to accelerate

bioprocess development and yield higher product titers.

Biography

Oliver Spadiut has completed his PhD in Biotechnology from BOKU University, Austria. He has done his Post-doctoral studies from KTH Royal Institute of

Technology, Stockholm, Sweden. Since 2010, he has been working as an Assistant Professor in Biochemical Engineering at TU Wien, Vienna, Austria. Currently,

he is the Principal Investigator of Integrated Bioprocess Development research group. He has published more than 60 papers in reputed journals and has been

serving as a reviewer for many journals.

oliver.spadiut@tuwien.ac.at

Oliver Spadiut, J Biotechnol Biomater 2017, 7:1(Suppl)

http://dx.doi.org/10.4172/2155-952X.C1.070