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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
Screening of petroleum degrading Bacillus spp. strains isolated from different non-contaminated soil
samples
Tuba Sevgi, Behice Zeren, Baran Enes Güler and Elif Demirkan
Uludag University, Turkey
Oil spill has become a global problem in industrialized and developing countries. Oil spills that occur during discharge from the
refineries, accidents of ships/tankers, their grounding, rupture on seabed and on shore pipelines, offshore oil production and
exploration platforms do affect these habitats causing irreversible damage to the biodiversity. The toxic effects of crude oil and refined
petroleum oils on plants, animals, humans and the environment are devastating. The aim of this study was to evaluate the potential
of petroleum hydrocarbon (PHC)-degrading 105 Bacillus spp. strains isolated from different non-contaminated soil samples. These
Bacillus spp. strains were screened for bacterial oil degradation using 3.5% petrol and 7 % diesel as sole carbon sources in Bushnell-
Haas agar medium. The plates were incubated at 37ºC for 7-17 days. After the incubation, only petroleum degrading bacteria
remained on the surface of the plates. Among the 105 Bacillus spp. strains, 22 Bacillus spp. strains were determined as potential
petroleum degrading strains. Most of these strains showed more degradation in diesel medium than petrol medium. Out of the
preselected 22 isolates, 18 isolates showed relatively high growth, while 4 others showed moderate to low cell counts after 7 or 17 days
of incubation period. This is the first study on Bacillus sp. strains isolated from Turkish soils. These isolates seemed to have potential
for bioremediation of oil contaminated soil and water.
Biography
Tuba Sevgi has completed her MSc from Technical University of Kaiserslautern in Molecular Biotechnology and Systems Biology, Germany. Currently, she is doing
her PhD in the Department of Biology, Faculty of Arts and Sciences, Uludag University. She is a Research Assistant in the Department of Biology, Faculty of Arts
and Sciences, Uludag University.
Tuba Sevgi et al., J Biotechnol Biomater 2017, 7:1(Suppl)