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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
Salt tolerance of potato: Genetically engineered with Atriplex canescens BADH gene driven by 3 copies of
CAMV35s promoter
Arfan Ali
University of Lahore, Pakistan
Potato (Solanum tuberosum L.) is ranked among the top leading staple food in the world. Salinity adversely affects potato crop
yield and quality. Therefore, increased level of salt tolerance is a key factor to ensure high yield. The present study focused on the
agrobacterium-mediated transformation of Atriplex canescens betaine aldehyde dehydrogenase (BADH) gene, using single, double
and triple CAMV35s promoter to improve salt tolerance in potato. Detection of seven potato lines harboring BADH gene, followed
by identification of T-DNA insertions, determination of transgenes copy no through Southern hybridization and quantification of
BADH protein through ELISA were considered in this study. The results clearly depicted that the salt tolerance of potato was found to
be promoter-dependent, as the potato transgenic lines with triple promoter showed 4.4 times more glycine betaine production which
consequently leads towards high resistance to salt stress as compared to transgenic potato lines with single and double promoters
having least production of glycine betaine. Moreover, triple promoter transgenic potato lines have also shown lower levels of H2O2,
malondialdehyde (MDA), relative electrical conductivity, high proline and chlorophyll content as compared to other two lines
having a single and double promoter. In silico analysis also confirmed that Atriplex canescens BADH has the tendency to interact
with sodium ions and water molecules. Taken together these facts, it can be concluded that over-expression of BADH under triple
CAMV35s promoter with more glycine betaine, chlorophyll and MDA contents, high relative quantities of other metabolites resulted
in an enhanced level of salt tolerance in potato.
Biography
Arfan Ali has completed his PhD from the University of the Punjab, Pakistan and Post-doctoral studies from the Centre of Excellence in Molecular Biology. Currently,
he is serving as an Assistant Professor at the University of Lahore, Pakistan. He has published more than 32 papers in reputed journals.
Arfan Ali, J Biotechnol Biomater 2017, 7:1(Suppl)