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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
Investigating miRNA-661 and ATG4b mRNA expression as potential biomarkers for hepatocellular
carcinoma
Osama Saber1 , Mahmoud A Ali1, Marwa Matboli2, Nashwa El-Khazragy 2, Nourhan Hossam2, Ahmed Hamdy1, Sarah El-Nakeep2, Ayman El-Sayed Shafei1 and
Randa Mostafa1
1Armed Forces College of Medicine, Egypt
2Ain Shams University, Egypt
Aim: In this study, we aimed to examine the statistical association of serum expression of miR-661 and ATG-4b mRNA with HCC
based on in silico data analysis followed by clinical validation.
Patients & Methods: Bioinformatics prediction was first applied to retrieve the potential miR serving as an epigenetic regulator of
ATG-4b mRNA. Real-time quantitative polymerase chain reaction( RT-qPCR) were used to examine the expression of miR-661
and candidate target gene ATG-4b mRNA in 105 hepatocellular carcinoma (HCC) patients, 50 chronic hepatitis C infected ( CHC)
patients and 45 healthy controls. The prognostic efficacy of the chosen genes was also explored.
Results: The expression of miR-661 and ATG-4B mRNA was positive in 97.14% and 77.14%, respectively. HCC patients with strong
discriminating power between HCC and control showed AUC=0.9 and 0.8, respectively. The median follow up period was 28 months.
The survival analysis showed that ATG-4b mRNA was not dependent on the prognostic factors. We also found that miR-661 was
positively correlated with ATG-4b mRNA in patients 'sera samples.
Conclusion: This is the first report about the considerable clinical use of miR-661 and ATG-4b mRNA in early detection and follow-
up of HCC patients.
Biography
Osama Saber is a second year Medical Cadet in Armed Forces College of Medicine (AFCM). He is a member of International Genetic Engineering Machine
Foundation. He has published many papers in various reputed journals.
Osama Saber et al., J Biotechnol Biomater 2017, 7:1(Suppl)