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Volume 7, Issue 1 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
March 20-21, 2017 Rome, Italy
&
15th World Congress on
2nd International Conference on
Biotechnology And Biotech Industries Meet
Enzymology and Molecular Biology
Enzymology & Mol. Biology 2017
Biotechnology Congress 2017
March 20-21, 2017
Halophilic microorganisms from mural paintings in old Romanian historical monument church and their
interactions with nanomaterials
Madalin Enache1, Roxana Cojoc1, Simona Neagu1 and Ioana Gomoiu2
1Institute of Biology Bucharest of the Romanian Academy, Romania
2Bucharest National University of Arts, Romania
The mural painting can be bio-deteriorated by micro-organisms in different ways depending on the taxonomic affiliation of micro-
organisms, their biology and succession while on a surface. The rate of bio-deterioration is dependent onmicroclimate conditions,
the chemical structure of deposits, the interrelation between bio-deteriogenes and some chemical processes. During colonization of
the mural painting surface, some species of micro-organisms synthesized pigments which could be released into the substrate or
remain localized within cells but the mural painting surface appears colored. The bio-pigmentation change esthetical appearance
of the mural painting or mortar where micro bio-deteriogenes develop. In our case studies (the refectory of Hurezi Monastery and
the pre-nave of Humor Monastery), the pink bio-pigmentation is the result of mural painting colonization by halophilic bacteria,
namely new strain of Garicola genus and some strains of Halobacillus spp. They have the ability to grow in media with negligible
salt concentration until saturation (extremely halophilic archaea). Taking into account the complexity of salt composition in mural
paintings, it appears that these could be a favorable environment from several moderately halophilic micro-organisms. On the other
hand, the investigated halophilic micro-organisms showed various growth answers when their culture medium was supplemented
with TiO2 nanoparticles. Such kinds of nanomaterials are currently investigated for their potential use in re-saturation procedures of
bio-deteriorated historical monuments.
Biography
Madalin Enache is working as a Principal Investigator in the field of Halophilic Microorganisms at the Institute of Biology, Bucharest of the Romanian Academy (IBB).
He Graduated from the University of Bucharest in Biochemistry field. Currently, he is also acting as Head of Microbiology Department of the IBB – coordinating
research and administrative activities of the Department of Microbiology (IBB); research activities in the fields of Microbiology, Biochemistry, Biology and Ecology.
He is involved in coordinating laboratory work, dissemination of the scientific results (scientific papers, participation to conferences and symposia – oral and posters
presentations), application for research projects, scientific reports and coordinating projects. He has expertise in various techniques of General Microbiology,
Microscopy, Biochemistry and Molecular Biology. His research topics include diversity and phylogeny of halophilic microorganisms; ecology of extremely halophilic
archaea, enzymology of halophilic microorganisms and; nanobiotechnology.
Madalin Enache et al., J Biotechnol Biomater 2017, 7:1(Suppl)