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.com
Volume 8
Journal of Environmental & Analytical Toxicology
EnviTox Summit 2018
September 17-18, 2018
September 17-18, 2018 Singapore
18
th
Global Summit on
Environmental Toxicology and Pharmacology
J Environ Anal Toxicol 2018, Volume 8
DOI: 10.4172/2161-0525-C2-015
Modulation of mercuric chloride by the combined treatment of N-acetyl cysteine and selenium: A
protective approach
Deepmala Joshi
1,2
, Sadhana Shrivastava
2
, Sangeeta Shukla
2
and Sunil Srivastav
1
1
Deen Dayal Upadhyaya Gorakhpur University, India
2
Jiwaji University, India
M
ercury is ubiquitous in the environment and is inevitable in both human and animals to avoid its exposure in toxic forms
of mercury on a regular basis. The channel news Asia, Singapore reported that 18 recalled beauty products contains
mercury and other potent ingredients exceeded permissible limit by more than 27,000 times. The aim of present study describes
the protective effect of selenium either alone or in combination with N-Acetyl Cysteine (NAC) against acute mercuric chloride
poisoning. The experiment was carried out in male albino Sprague Dawley rats (n=30) which was divided into 5 groups. Group
1 served as control. Groups 2-5 were administered mercuric chloride (HgCl
2
: 12 μmol/kg, i.p.) once only, group 2 served as
experimental control. Animals of groups 3, 4 and 5 were received N-acetyl cysteine (NAC: 0.6 mg/kg, i.p.) and selenium (Se:
0.5 mg/kg, p.o.) and NAC with Se in combination. Acute HgCl
2
toxicity caused significant rise in serum AST, ALT, SALP,
LDH, albumin, bilirubin, GGT, TG, cholesterol, protein, urea, creatinine, uric acid and BUN content. Animals of toxicant
exposed group also showed significantly higher mercury content in liver and kidney, significant rise in lipid peroxidation level
with concomitant decrease in reduced glutathione content and the antioxidant enzyme activities of superoxide dismutase and
catalase after HgCl
2
exposure. Results of the present investigation clearly showed that the combination therapy with NAC+ Se
provides maximum protection against mercury toxicity than mono-therapy by preventing biological membrane from oxidative
damage and metal mediated free radical attacks.
deepmalajoshi953@gmail.com