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Volume 8 Journal of Environmental & Analytical Toxicology EnviTox Summit 2018
September 17-18, 2018
September 17-18, 2018 Singapore
18th Global Summit on
Environmental Toxicology and Pharmacology
Carlos Medina Saldivar et al., J Environ Anal Toxicol 2018, Volume 8
DOI: 10.4172/2161-0525-C2-015
Post-harvest evaluation of dimethoate, chlorothalonil and chlorpyrifos by CG-ECD in Peruvian
varieties of Chenopodium quinoa Willd
Fredy Enrique Quispe Jacobo, Carlos Medina Saldivar and Chirinos Pajuelo Diego
National Institute of Agrarian Innovation, Peru
Chenopodium quinoa is an important nutritional Andean grain produced by farmers in Peruvian highlands and Plateau.
In the last years, new crops like quinoa in Peruvian coast have been cultivated due to increasing of worldwide demand
of quinoa . However, the growing under this agro climatic condition, quinoa was more sensitive to plague attacks and fungus
infection, for those reasons farmers increasing the use of pesticides, which in many cases exceeded the maximum residue
limits established by international organizations in food and feed. The purpose of this study was the post-harvest evaluation
of dimethoate, chlorothalonil and chlorpyrifos by Gas Chromatography coupled with Electron Capture Detector (GC-ECD)
using a solid phase extraction in seeds of four varieties of Peruvian quinoa. The study began with the development, optimization
and validation of the method for determining the levels of dimethoate, chlorothalonil and chlorpyrifos in the post-harvest
stage of Salcedo, Quillahuaman, Santa Ana and Altiplano quinoa varieties, which were harvested in conventional and organic
field at the central coast of Peru (Lima). Validation by CG-ECD shows pesticides recovery percentages between 98.98 and
118.44% with relative standard deviation up to 10.5%, significant correlation coefficients (≥0.9979) and limits of quantification
(≥5.402 ppb) were observed in the quinoa seeds, which meet the standard validation parameters of the European commission.
The evaluation of chlorothalonil in Quillahuman and Santa Ana at conventional field exceeded the maximum residue level
allowed, however three months later the concentration of the pesticides used in the culture decreased significantly. In the case
of organic field the pesticides were not observed. The gas chromatography coupled with electron capture detector can be used
to determine the levels of dimethoate, chlorothalonil and chlorpyrifos in quinoa seeds. Recommendations are to determine the
levels of these pesticides in the Peruvian market.
References
1. Liu Z, Qi P, Wang X, Wang Z, Xu X, Chen W, et al. (2017) Multi-pesticides residue analysis of grains using modified
magnetic nanoparticle adsorbent for facile and efficient cleanup. Food Chemistry; 230: 423-431.
2. Ortega Barriga R E, Zamallora Cuba W A, Tornisielo V L, Zirena Vilca F (2016) Determination of organo-chlorine
pesticides in organic quinoa grains (Chenopodium quinoa Willd) by GC-μECD, using the QuEChERS method. Revista de
Investigación Altoandina; 18 (1): 19-26.
Biography
Fredy Enrique Quispe Jacobo is a researcher from National Institute of Agrarian Innovation (INIA). He has expertise in Researching and Technological Development
(RTD) of Natural and Agro industrial products. He is a leader of Nutritional Area from Genetic Resources and Biotechnology Division at INIA, and with his team
executes Projects to know the nutritional contents, minerals, and functional properties of genetic resources, likewise pesticide residuals used in the production at
field.