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conferenceseries
.com
Volume 8
Journal of Environmental & Analytical Toxicology
EnviTox Summit 2018
September 17-18, 2018
September 17-18, 2018 Singapore
18
th
Global Summit on
Environmental Toxicology and Pharmacology
J Environ Anal Toxicol 2018, Volume 8
DOI: 10.4172/2161-0525-C2-015
A novel high performance liquid chromatographic method for determination of ceftriaxone in
forensic matrices
Jaskaran Singh
Amity University, India
Lovely Professional University, India
A
n isocratic liquid chromatographic method with UV detection at 242 nm is described for determination of ceftriaxone.
Chromatographic separation of drug was achieved on a hypersil ODS C-18 150*4.6 mm, 5 μm column using a mobile
phase consisting of a binary mixture of phosphate buffer (4.5590 gm of potassium dehydrogenate orthophosphate in 1.0 liter
water and pH was adjusted to 7.5) and methanol in the ratio of 60:40 v/v. The developed liquid chromatographic method offers
symmetric peak shape, good resolution and reasonable retention time for drug. Linearity, accuracy and precision were found
to be acceptable over the concentration range of 10-40 microgram/ml for ceftriaxone. The LC method can be used for the
detection of ceftriaxone over dosage cases found in various forensic matrices like blood, gastric lavage, viscera and for quality
control of formulated products.
jaskaransingh630@gmail.com