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.com
Volume 8
Journal of Environmental & Analytical Toxicology
EnviTox Summit 2018
September 17-18, 2018
September 17-18, 2018 Singapore
18
th
Global Summit on
Environmental Toxicology and Pharmacology
Paula Moyano et al., J Environ Anal Toxicol 2018, Volume 8
DOI: 10.4172/2161-0525-C2-015
Primary hippocampal neuronal cell death induction after acute and repeated Paraquat exposures
mediated by glutamatergic transmission disruption
Paula Moyano
1
, Javier Del Pino
1
, Matilde Ruiz
1
, María Jesús Díaz
1
, Gloria Gomez
1
, María José Anadón
1
, Margarita Lobo
1
, José Manuel Garcia
1
, María
Teresa Frejo
1
and Jimena García
2
1
Universidad Complutense Madrid, Spain
2
Universidad Alfonso X El Sabio, Spain
P
araquat (PQ) is a widely used non-selective contact herbicide shown to produce memory and learning deficits after
acute and repeated exposure similar to those induced in Alzheimer’s Disease (AD). However, the complete mechanisms
through which it induces these effects are unknown. On the other hand, glutamatergic systems, mainly in the hippocampus
are involved on learning, memory and cell viability regulation. An alteration of hippocampal glutamatergic transmission or
neuronal cell loss may induce these effects. In this regard, it has been suggested that PQ may induce cell death and affect
glutamatergic transmission, which alteration could produce neuronal loss. According to these data, we hypothesized that PQ
could induce hippocampal neuronal loss through glutamatergic transmission alteration. To prove this hypothesis, we evaluated
in hippocampal primary cell culture, the PQ toxic effects after 24 hours and 14 consecutive days exposure on neuronal viability
and the glutamatergic mechanism related to it. This study shows that PQ disrupted glutamate levels through induction of
glutaminase activity. In addition, PQ induced, after 24 hours and 14 days exposure, cell death on hippocampal neurons that
was partially mediated by glutamatergic transmission disruption. Our present results provide new view of the mechanisms
contributing to PQ neurotoxicity and may explain cognitive dysfunctions observed after PQ exposure.
Biography
Paula Moyano has completed her JD degree from the University Complutense, the University of Madrid in 2013. She has a Masters in Pedagogical Sciences. She
is specialized in neuro-toxicology and legal sciences and has completed her PhD in Toxicology and Legal Medicine.
pmoyanocires@ucm.es