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Volume 8 Journal of Environmental & Analytical Toxicology EnviTox Summit 2018
September 17-18, 2018
September 17-18, 2018 Singapore
18th Global Summit on
Environmental Toxicology and Pharmacology
Javier Del Pino et al., J Environ Anal Toxicol 2018, Volume 8
DOI: 10.4172/2161-0525-C2-015
Amitraz changes 5-HT levels mediated by alterations in estradiol content in CNS of male rats
Javier Del Pino1, Matilde Ruiz1, María Jesús Díaz1, Gloria Gomez1, María José Anadón1, Margarita Lobo1, José Manuel Garcia1, María Teresa Frejo1 and
Jimena García2
1Complutense University of Madrid, Spain
2Universidad Alfonso X El Sabio, Spain
Amitraz is a formamidine insecticide/acaricide that alters different neurotransmitters levels, among other neurotoxic
effects. Oral amitraz exposure (20, 50 and 80 mg/kg bw, 5 days) has been reported to increase Dopamine (DA) content
and to decrease its metabolites and turnover rates in the male rat brain, particularly in the striatum, prefrontal cortex and
hippocampus. However, the mechanisms by which these alterations are produced are not completely understood. Amitraz
alters estradiol concentrations in the brain that regulate the enzymes responsible for this neurotransmitter synthesis and
metabolism. Thus, alterations in estradiol levels in the brain could mediate the observed effects. To test these hypothesis
regarding possible mechanisms, we treated male rats with 20, 50 and 80 mg/kg bw for 5 days with or without tamoxifen (TMX, 1
mg/kg bw), a selective estrogen receptor antagonist and then isolated tissue from striatum, prefrontal cortex and hippocampus.
We then measured tissue levels of DA neurotransmitter. Amitraz produced a dose-dependent increase of the DA levels in all
brain regions studied compared to the control group. The increase in DA ranged from highest to lowest in prefrontal cortex,
striatum and hippocampus. Moreover, amitraz induced a dose-dependent decrease of DOPAC and HVA metabolites content
and turnover rate (DOPAC+HVA/DA) in all brain regions studied compared to the control group. There were no differences
between the decrease in the DOPAC and HVA content in the hippocampus, but the decrease in the DOPAC content was
higher than the decrease in the HVA content in the striatum and the decrease in the HVA content was higher than the decrease
in the DOPAC content in prefrontal cortex. The decrease of DA turnover rate (DOPAC+HVA/DA) ranged from highest to
lowest in prefrontal cortex, hippocampus and striatum. TMX co-treatment with amitraz partially reversed the change in DA
neurotransmitter and its metabolites levels as well as the turnover rates induced by amitraz alone in all brain regions studied.
Our present results provide new understanding of the mechanisms contributing to the harmful effects of amitraz.
Biography
Javier Del Pino has completed his Doctor of Pharmacy degree from the Complutense University of Madrid in 2004. He has completed his Master’s degree in
Science, specialized in Neurotoxicology and Neurodevelopmental Toxicology and has completed his PhD in Toxicology. He has worked in the Institute of Health
Carlos III from the National Center of Environmental Health. He was Associated Researcher at University of Massachusetts (UMASS) working in Sandra Petersen’s
Lab in a National Institute of Health (NIH) project on developmental effects of TCDD endocrine disruptor on sexual differentiation. In 2016 he got a position as
Associated Professor of Toxicology at the Complutense University of Madrid.