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Volume 7, Issue 5 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biotechnology 2017
November 13-14, 2017
November 13-14, 2017 Osaka, Japan
19th World Congress on
Biotechnology
Cloning and sequence analysis of bZIP and WRKY transcription factor genes in EinkornWheat (Siyez)
Pinar Baloglu and Mehmet Cengiz Baloglu
Kastamonu University, Turkey
Wheat is an annual herbaceous plant whose breeding is done all over the world. Siyez wheat (Tiriticum monococcum) with
2n chromosomes is the first wheat variety which is known the oldest and taken culture. bZIP transcription factors have
DNA binding motifs and form the leucine zipper dimerization. Most of WRKY type transcription factors control regulation of
important functions for the development of plants. In this study, transcription factor genes were firstly cloned form Einkorn
wheat. For this, genes were amplified in PCR using gene specific primers with Pfu Taq Polymerase enzyme. PCR products were
transferred to pENTR™/D-TOPO® input vector. Plasmids containing genes were sent to sequencing. Finally, the sequences
of the genes were aligned using CLC Genomics Workbench bioinformatics program. bZIP gene with 450 bp in length and
WRKY gene with 672 bp in length were successfully cloned in a Gateway-compatible input vector. DNA sequence of genes was
translated to protein sequence. A total of 150 amino acids in length for bZIP proteins and a total of 224 amino acids in length
for WRKY proteins were determined. 3-D structure of proteins was modeled using Phyre2 program. The structural differences
of these proteins in Siyez were identified. With this study, the sequence of bZIP and WRKY type transcription factors genes
were firstly determined in ancestral wheat cultivar, Siyez and tried to obtain information about the function of these genes.
Obtained results from this study may be used for development of abiotic stress resistance plants.
Biography
Pinar Baloglu is a Biologist at Kastamonu University in Research and Application Center. She has received her MSc at Department of Genetic and Bioengineering
from Kastamonu University. In her thesis, she has identified 79 LEA (Late Embryogenesis Abundant) genes in cucumber genome. She has published 2 manuscripts
on topics concerning with gene families identification in different plants. She has also attended many congress related with Molecular Biology and Bioinformatics.
Presently, she is a PhD candidate in same department.
Pinar Baloglu et al., J Biotechnol Biomater 2017, 7:5 (Suppl)
DOI: 10.4172/2155-952X-C1-082