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Volume 7, Issue 5 (Suppl)

J Biotechnol Biomater

ISSN: 2155-952X JBTBM, an open access journal

Biotechnology 2017

November 13-14, 2017

November 13-14, 2017 Osaka, Japan

19

th

World Congress on

Biotechnology

Effect of water and super absorbent polymer (SAP) content on the efficiency of production biodiesel

by liquid enzyme catalyst

Dinh Thi My Huong and Chia-Hung Su

Ming Chi University of Technology, Taiwan

W

ater is the factor need to attend on the esterification to produce biodiesel by enzyme catalysts because of its complicated

influences on the catalyst activity and stability of enzyme as well as the productivity of reaction. Some studies conclude

that some lipases need to be maintained for its proper structure by the certain amount of water. On the other hand, water is the

by-product of the ester reaction, hence the high-water content exists in the reaction media causes the back-forward reaction,

consequences to reduce the yield. Therefore, this study drives to identify the suitable amount of water for assisting activation of

liquid enzyme catalyst and investigate the using SAP to absorb the produced water. The effect of water content on the enzyme

activity was assigned through discussed the efficiency of the esterification with molar ratio of oleic acid to methanol 1:3 at the

temperature of 25

o

C, catalyst amount of 10 wt.% and varied water amount of 0 to 30 wt.% (base on the fatty acid mass) for 1

hour and 3 hours. Another reaction was carried out with the condition similar to above reactions and added 5% (w/w oleic

acid) of SAP amount to study the ability of maintaining the esterification. Results illustrated that the conversion was increased

obviously from 0% to 95 % with added water content increased from 0 wt.% to 30 wt.%. Meanwhile the conversion rate was

improved significant with using SAP, the yield reached to 95% with added water amount of 10 wt.% for only 1 hour.

Biography

Dinh Thi My Huong has completed her Bachelor’s degree from Da Nang University of Technology, recently pursued Master’s program of Biochemistry at from Ming

Chi University of Taiwan. She is currently the Lecturer of Da Nang College of Technology.

myhuongdinh@gmail.com

Dinh Thi My Huong et al., J Biotechnol Biomater 2017, 7:5 (Suppl)

DOI: 10.4172/2155-952X-C1-082