biotechnology-2017-scientifictracks-abstracts

16 / 16

Page 35
conferenceseries.com
Volume 7, Issue 5 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biotechnology 2017
November 13-14, 2017
November 13-14, 2017 Osaka, Japan
19th World Congress on
Biotechnology
Effect of surfactants on the production and structure of curdlan from Agrobacterium sp. ATCC 31749
Xiaobei Zhan, Ying Liang and Li Zhu
Jiangnan University, China
The serious envelopment of curdlan on Agrobacterium sp. ATCC 31749 during fermentation is a major obstacle to increase
curdlan production and the compact macrostructure of curdlan produced from Agrobacterium sp. ATCC 31749 limits
its application in preparing curdlan β-glucooligosaccharides. In this study, the effects of different surfactants on curdlan
production by Agrobacterium sp. ATCC 31749 was investigated and several new perspectives concerned with the effect of
Tween 80 promoting curdlan production and altering curdlan structure were presented. Maximum curdlan production (51.94
g/L) was achieved when 16 g/L Tween-80 was added at the beginning of the cell growth stage. The addition of Tween-80 at
higher concentration inhibited cell growth. However, the addition of 16 g/L Tween-80 enhanced the production of curdlan
with a looser ultrastructure, significantly weakened the envelopment of curdlan on Agrobacterium sp. ATCC 31749, altered
the fine structure of cell membrane and increased the cell membrane permeability. Compared with commercial curdlan, the
curdlan with a looser ultrastructure exhibited higher substrate-binding affinity and maximum reaction rate when it was used as
the substrate for preparing curdlan β-glucooligosaccharides. These findings demonstrate the mechanisms by which Tween-80
enhances curdlan production and provide a cheap and feasible approach to weaken the envelopment of water-insoluble
polysaccharides on bacteria. Meanwhile, this paper provides an effective method to produce an ideal substrate so as to prepare
oligosaccharides using enzymic degradation.
Biography
Xiaobei Zhan has completed his PhD from Kansans State University in 2004 and is a Professor of Fermentation Engineering from Jiangnan University. He is the
Director of Industrial Microbiology and Biological Reaction Engineering Research Center, School of Biotechnology, Jiangnan University and the deputy Director of
Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education. He is also the ASAE and IFT Institute of United States. His research focus
is Technology of high-viscosity fermentation, biosynthesis of microbial polysaccharide, preparation of functional oligosaccharide and design of bioreactor. He has
published more than 30 papers in reputed journals.
Xiaobei Zhan et al., J Biotechnol Biomater 2017, 7:5 (Suppl)
DOI: 10.4172/2155-952X-C1-082