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Volume 7, Issue 5 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biotechnology 2017
November 13-14, 2017
November 13-14, 2017 Osaka, Japan
19th World Congress on
Biotechnology
CRISPR-PCS: An efficient and versatile chromosome splitting technology in Saccharomyces cerevisiae
Saeed Kaboli
Shahid Beheshti University, Iran
PCR-mediated chromosome splitting (PCS) was developed in the yeast Saccharomyces cerevisiae. It is based on homologous
recombination and enables division of a chromosome at any point to form two derived and functional chromosomes.
However, because of low homologous recombination activity, PCS is limited to a single site at a time, which makes the
splitting of multiple loci laborious and time-consuming. Here we have developed a highly efficient and versatile chromosome
engineering technology named CRISPR-PCS that integrates PCS with the novel genome editing CRISPR/Cas9 system. This
integration allows PCS to utilize induced double strand breaks to activate homologous recombination. CRISPR-PCS enhances
the efficiency of chromosome splitting approximately 200-fold and enables generation of simultaneous multiple chromosome
splits. We propose that CRISPR-PCS will be a powerful tool for breeding novel yeast strains with desirable traits for specific
industrial applications and for investigating genome function.
Biography
Saeed Kaboli has completed his PhD and Postdoctoral degrees in Department of Biotechnology, Osaka University, Japan. Presently, he is a Postdoctoral
Researcher in Sciences and Biological Technologies, Shahid Beheshti University, Iran. Also, he is engaged in a project entitled “Development of novel genome
engineering technology and its application in bioscience and biotechnology”.
Saeed Kaboli, J Biotechnol Biomater 2017, 7:5 (Suppl)
DOI: 10.4172/2155-952X-C1-082