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Volume 7, Issue 5 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biotechnology 2017
November 13-14, 2017
November 13-14, 2017 Osaka, Japan
19th World Congress on
Biotechnology
Bioactivity of different bee honey samples
Mahasin Ahmed Wadi
Princess Nourah Bint Abdulrahaman University, KSA
Background: The therapeutic value of honey was underlined in various literatures. Honey was widely used in folk medicine
throughout the world. Honey has been used to treat a number of clinical conditions: Treatment of burns, wounds, peptic ulcers,
gastritis, eye infection and sore throat. Antibacterial activity is attributed partially to the high osmolality of the sugar content of
honey. Additional antibacterial activity of honey was investigated by extraction and fractionation of honey by organic solvents.
Honey ethyl acetate extract revealed potent antibacterial activity.
Objectives: The objective of the current prospective study is to determine the antimicrobial activity of fifteen (15) different
honey samples collected from different floral origin and to verify the nature of active fraction of bee honey.
Methods: Fifteen different raw bee honey samples were obtained from different countries, as well as commercially sold honey
samples from the local market different brands, of different floral origin. In vitro antibacterial activity of bee honey, petroleum
ether, diethyl ether and ethyl acetate extracts of honey samples were tested against five standard organisms; Staphylococcus
aureus: ATCC 29213, Staphylococcus Methicillin Resistant (MRSA), ATCC: 23591 Escherichia coli: ATCC 25922 Klebsiella
pneumoniae: ATCC 700603 and Pseudomonas aeruginosa : ATCC 27853.
Results: All honey samples exerted inhibitory effects on both Gram-positive and Gram-negative organisms. The petroleum
ether and diethyl ether fraction exerted no activity, while the aqueous residue exerted strong antibacterial activity. Ethyl acetate
fraction showed strong antibacterial activity.
Conclusion: The findings of the current study confirmed that all honey samples exerted strong antibacterial activity. The
chemical findings of this study indicate the presence of polar antibacterial agent(s) which is characterized by its extractability
by ethyl acetate to the organic phase.
Biography
Mahasin Ahmed Wadi has completed her PhD in Medical Microbiology and is currently working at College of Nursing, Princess Nourah Bint Abdulrahaman
University, Riyadh, Saudi Arabia. She has published a numbers of papers in reputed journals and participated in many internationals and national conferences. She
issued a patent research about the antimicrobial activity of Sudanese bee honey. She is a Member of many international associations: German Apitherapy Society,
American Apitherapy Society, International Bee Research Association, European Society of Clinical Microbiology and Infectious Disease ESCMID. She has served
as a Reviewer of various journals and has her research interest in the antibacterial and haling effects of Bee honey as natural product.
Mahasin Ahmed Wadi, J Biotechnol Biomater 2017, 7:5 (Suppl)
DOI: 10.4172/2155-952X-C1-082