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Volume 8 Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Biotech Congress 2018 & Enzymology 2018
March 05-07, 2018
JOINT EVENT
20th Global Congress on Biotechnology
3rd International Conference on Enzymology and Molecular Biology
&
March 05-07, 2018 London, UK
Efficiency of the antioxidative system is the first prerequisite for effective doubled haploids production
with the use of isolated microspore culture method
Iwona Żur1, Jozsef Fodor2, Katarzyna Gawrońska3, Gabriela Gołębiowska-Pikania3, Katarzyna Juzoń1, Przemysław Kopeć1, Ewa Surówka1, Franciszek
Janowiak1, Monika Krzewska1, Ewa Dubas1 and Balazs Barna2
1Polish Academy of Sciences, Poland
2Hungarian Academy of Sciences, Hungary
3Pedagogical University of Kraków, Poland
The technology of doubled haploids as the fastest route to total homozygosity is highly appreciated inmany domains of basic research
and breeding. Among several methods, the one using isolated and in vitro-cultured immature cells of male gametophyte induced
towards embryogenic development (microspore embryogenesis-ME) possesses the highest potential for commercial application.
However, efficient ME induction requires a precisely balanced stress treatment, strong enough to induce microspore reprogramming
but not exceeding cell stress tolerance threshold. As the general cause of injuries in in vitro-cultured cells is the overproduction of
reactive oxygen species (ROS), an efficient antioxidative defence was suggested as the first prerequisite for stress survival and effective
ME initiation. To establish the role of ROS and the antioxidative system in ME initiation, the generation of hydrogen peroxide, and
the activities of antioxidative enzymes and low molecular weight antioxidants were analysed in isolated microspores of two cultivars
of barley (Hordeum vulgare L.), winter cv. Igri and spring cv. Golden Promise, differing significantly with respect to embryogenic
potential. The analyses were conducted in microspores redirected towards embryogenic development by low temperature tillers pre-
treatment (4 weeks at 4°C). Additionally, the effects of compounds known as cellular redox status modifiers, e.g. glutathione and L-2-
oxo-4-thiazolidinecarboxylic acid (OTC), on microspore viability and ME initiation efficiency were estimated. The received results
suggest that the activity of the antioxidative system is the first prerequisite for successful ME initiation, though in the case of its low
activity, antioxidative defence could be supported by the application of exogenous antioxidants.
Biography
Iwona Żur completed her PhD and Habilitation in the field of Agronomy and Plant Physiology at the University of Agriculture in Kraków, Poland. Since 2010, she has
been the Head of the Department of Cell Biology at the Institute of Plant Physiology Polish Academy of Sciences. She has published 38 papers in peer-reviewed
journals.
Iwona Żur et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C2-091