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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Biotech Congress 2018 & Enzymology 2018
March 05-07, 2018
JOINT EVENT
20
th
Global Congress on
Biotechnology
3
rd
International Conference on
Enzymology and Molecular Biology
&
March 05-07, 2018 London, UK
Enhanced production of
Bacillus thuringiensis
subspecies israelensis delta endotoxin by the use of rotten
pineapple juice and fish-amino acid as medium ingredients
C Gopinathan
and
Sonia Sharma
University of Calicut, India
M
osquito borne diseases not only cause loss of lives but also impose heavy health and economic burdens. Extensive use of
chemical insecticides for the control of malaria and other mosquito borne diseases has led to the development of resistance in
mosquitoes to these insecticides and are hazardous to the environment. Biolarvicides of the strain
Bacillus thuringiensis
israelensis
(Bti), serotype H-14 is highly effective against mosquito larvae. Even though Bti products are efficient controls for mosquito and black
fly larvae, their use in developing countries is limited by their cost. Thus, there is a need to reduce the overall production cost of Bti in
order to make it competitive in the market. It depends on many factors; however, the raw material cost is one of the most important
criteria which may comprise >70% of the overall production cost. Fruit wastes are available in plenty and contain mainly fructose as
the carbon source, which is easily fermentable and can substitute costly substrates like glucose. Channelizing huge quantities of rotten/
waste pineapples which otherwise are discarded can substantially reduce production cost of Bti. Similarly fish-amino acid produced
by fermenting rotten fish and jaggery/molasess has proved to be excellent as a medium supplement; especially to overproduce the
much wanted delta endotoxin produced by Bti. India is one of the countries leading in fruit and vegetable production. It is also blessed
with one of the longest coastline in the world of approximately 7516.6 km. The total annual catch is around 4 million metric tons. In
addition it is second after Brazil in sugarcane cultivation with an annual yield of 3412 million metric tons. The massive availability
of fruit wastes (pineapples) and huge quantities of rotten /discarded fish , which are freely available, all can be channelized for cost
effective production of this value added product, substantially lowering the media cost of Bti production when scale- up is attempted.
Results show biomass increase of up to 27% compared to control when pineapple juice was used as the main carbon source. The
toxicity improvements with fish-amino acid supplemented medium, shows considerable reduction in killing time of
Aedes aegypti
larvae.
Biography
C Gopinathan is working as a Associate Professor in the Department of Biotechnology at the University of Calicut. He has finished his MSc, MTech in Biotechnology.
His specialization is towards Bioprocess Technology/Fermentation Technology. He is the former member of Academic Council, University of Calicut, American
Society for Microbiology and Association of Microbiologists of India.
shinuu@rediffmail.comC Gopinathan et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C2-091