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Volume 8, Issue 6 (Suppl) J Bioequiv Availab
ISSN: 0975-0851 JBB, an open access journal
Page 30
Notes:
Biopharma 2016
September 14-16, 2016
conferenceseries.com
September 14-16, 2016 San Antonio, USA
2nd International Conference & Expo on
Biopharmaceutics and Biologic Drugs
Protective efficacy of Emblica officinalis Linn. against radiation and lead induced qualitative, quantitative
and biochemical alterations in mouse testes
R K Purohit, Aruna Chakrawarti, Manisha Agarwal and K M Bhartiya
Govt. Dungar College, India
In today’s changing global scenario, ionizing radiation is considered as most potent cause of oxidative stress mediated by free
radical flux which induces severe damage at various hierarchical levels in the organization in the living organisms. Testis is a highly
prolific tissue with fast cellular renewal and poor antioxidant defense; therefore, it becomes an easy target for the radiation- induced
free radicals that have long been suggested as major cause of male infertility. Radiation causes deleterious effects in all forms of
life due to increasing utilization and production of modern technology, a simultaneous exposure of organisms to heavy metals is
also unavoidable. These heavy metals become toxic when present in large quantities, with increasing the industrial revolution and
industrial waste, the emission of lead has increased into the environment. Thus concomitant exposure to lead acetate and ionizing
radiation might produce deleterious effect upon biological system. The total environmental burden of toxicants may have greater
effect as against their individual impact as expected by their nature. So interaction between radiation and other toxicants represents a
field of great potential importance. In the recent years, immense interest has been developed in the field of chemoprotection against
radiation and heavy metals induced changes. In view of the potential for practical application, a variety of compounds are being
tested for their radioprotective activities. Among these, Emblica holds a great promise. In light of the above, the present study was
aimed to evaluate the protective effect of Emblica officinalis extract (ECE) against radiation and lead induced qualitative, quantitative
and biochemical alterations in the testes of Swiss albino mice. The animals were exposed to 3.0 and 6.0 Gy of gamma rays with or
without lead acetate treatment. The Emblica was administered seven days prior to irradiation or lead acetate treatment. The animals
were divided into seven groups. The nondrug treated control groups were administered lead acetate and exposed to irradiation
whereas the experimental groups were given Emblica seven days prior to irradiation or lead acetate treatment. Irradiation resulted
into significant decrease in the frequency of different spermatogenic cell counts along with severe histo-pathological lesions up to 14th
day in control animals and day-14 in experimental animals thereafter, recovery followed towards the normal architecture. ECE pre-
treatment effectively prevented radiation induced end of experimentation. Furthermore, ECE administration inhibited radiation and
lead induced changes in the testes of mice. These observations signify that the Emblica officinalis extract can be used as an efficient
radio- protector against radiation mediated qualitative, quantitative and biochemical alterations in testes.
Biography
R K Purohit at present is working as Professor of Zoology in Govt. Dungar College, Bikaner (Rajasthan), India. He has 25 five years of teaching and research
experience. He has published around 40 research papers in journals of international repute. He has attended dozens of national and international conferences
and also presented his papers. He has also chaired the sessions in many international conferences. He has produced 19 PhDs and 20 MPhil scholars under his
able supervision. He has visited Singapore, Malaysia and Japan. He is the recipient of many national and international awards. He has organized one “National
Conference on Herbal Radioprotection” in the year 2004 (October, 2004) and an International conference in January 2012. He is the life member of many academic
societies. At present, he is holding the prestigious status of National Secretary, Indian Society for Radiation Biology who is specially working in the field of herbal
radioprotection.
R K Purohit et al., J Bioequiv Availab 2016, 8:6 (Suppl)