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conferenceseries
.com
Volume 9
Journal of Bioremediation & Biodegradation
ISSN: 2155-6199
Biofuel Congress 2018 &
Biomass 2018
September 04-06, 2018
JOINT EVENT
September 04-06, 2018 | Zurich, Switzerland
13
th
Global Summit and Expo on
Biomass and Bioenergy
&
12
th
World Congress on
Biofuels and Bioenergy
Improved Production of Cellulosic Bioethanol using
Miscanthus
Hydrolysate by Engineered
Saccharomyces cerevisiae
Young-Lok Cha
1
, Yong-ho Moon
2
, Kwang-soo Kim
3
, Ji-eun Lee
4
, Da-eun Kwon
5
and
Yong-ku Kang
6
1,2,3,4,5,6
Bioenergy Crop Research Institute, National Institute of Crop Science, Republic of KOREA
I
n order to industrialize bioethanol based on cellulosic biomass, securing economic efficiency is very important. Until
now, fermentation using glucose converted from cellulose through pretreatment and saccharification process was mainly
performed, resulting in economically lower efficiency. The industrialization of strains capable of metabolizing xylose produced
from cellulosic biomass was insufficient. Recently, an engineered
Saccharomyces cerevisiae
was developed for the effective
bioethanol production. In this study, the yield of ethanol production was investigated using engineered
Saccharomyces cerevisiae
capable of xylose metabolism. The raw materials was pretreated with a twin-screw extrusion reactor under conditions: 0.5 M
NaOH 27 L/h, biomass feeding 4.5 kg/h at 99℃. The production of substrates for the ethanol fermentation was produced 120 g /
L of glucose and 40 g / L of xylose through alkaline pretreatment and saccharifica-tion. In result, 65 g/L of ethanol in 48 h from
Miscanthus
hydrolysate was obtained using engineered
Saccharomyces cerevisiae
capable of xylose metabolism. In conclusion,
the yield of ethanol production was improved 40% from 46 g/L to 65 g/L with same substrate.
Recent Publications
1. Young-lok Cha, Jungwoo Yang, Yuri Park, Gi Hong An, Jong-woong Ahn, Youn-ho Moon, Young-mi Yoon,
Gyeong-dan u, In-hu Choi (2015) Continuous alkaline pretreatment of
Miscanthus
sacchariflorus using a bench-
scale single screw reactor. Bioresource Technology 181:338-344.
2. Young-lok Cha, Jungwoo Yang, Sun-il Seo, Gi Hong An, Youn-ho Moon, Gyeong-dan Yu, Ji-Eun Lee, Jong-woong
Ahn, Kyeong-Bo Lee(2016) Alkaline twin-screw extrusion pretreatment of
Miscanthus
with recycled black liquor
at the pilot scale. Fuel 164:322-328.
3. Yamada R., Taniguchi N., TanakaT., Ogino C., Fukuda H., Kondo A. (2011) Direct ethanol production from
cellulosic materials using a diploid strain of
Saccharomyces cerevisiae
with optimized cellulose exprestion.
Biotechnol. Biofuels. 4:1-9.
4. Kim S. R., Park, Y.C., Jin Y.S., Seo J.H. (2013) Strain engineering of
Saccharomyces cerevisiae
for enhanced xylose
metabolism. Biotechnol. Adv. 31:851-861.
5. Ye-Gi Lee, Yong-su Jin, Young-lok Cha, Jin-ho Seo (2017) Bioethanol production from cellulosic hydrolysates by
engineered industrial
Saccharomyces cerevisiae
. Bioresocurce Technology 228:355-361.
Biography
Young-Lok Cha was a doctorate at the University of Hannover in Germany. He is working as a senior researcher at the National Institute of Crop Science, Rural
Development Administration in Korea and his major is the development of lignocellulosic biomass conversion technology and biofuels production at pilot scale.
biocha@korea.krYoung-Lok Cha et al., J Bioremediat Biodegrad 2018, Volume 9
DOI: 10.4172/2155-6199-C1-015