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Volume 9
Journal of Bioremediation & Biodegradation
ISSN: 2155-6199
Biofuel Congress 2018 &
Biomass 2018
September 04-06, 2018
JOINT EVENT
September 04-06, 2018 | Zurich, Switzerland
13
th
Global Summit and Expo on
Biomass and Bioenergy
&
12
th
World Congress on
Biofuels and Bioenergy
Capillary electrophoresis in the analysis of volatile fatty acids, ammonium, K, Ca and Mg from
mesofilic anaerobic digestion samples
Jasmiina Haverinen
1
Mari Jaakkola
2
,
and
Tiina Tolonen
3
1
University of Oulu, Finland
2
Kajaani University Consortium, Finland
3
Unit of Measurement Technology, Finland
Statement of the Problem:
Biogas process monitoring help to maintain stable process and costs of basic monitoring are often
much lower than the costs with re-establishing a destabilised plant. Reactor acidification through reactor overload is one of
the most common reasons for process deterioration in anaerobic digesters (ADs): A build-up of volatile fatty acids (VFAs)
decrease pH and result in toxic conditions. Ammonia ensure supply of nitrogen as nutrient for biomass and increase system's
bu er capacity, but elevated NH4 concentration causes toxic e ects. To obtain accurate monitoring results, sampling, sample
pre-treatment need to be controlled. This may be a challenge when plant is located far away from the laboratory. In this study, a
capillary electrophoresis (CE) was utilized in the analysis of individual VFAs, ammonium, and nutrients (K, Ca, Mg) from the
AD samples obtained from biogas factory. On-site monitoring of the analytes was performed to obtain fast analysis and minor
sample storage and transportation times. In addition, longer chain fatty acids and bacterial profiles of the samples were studied.
Methodology & Theoretical Orientation:
Samples were collected in biogas plant consisting three separate mesofilic AD
reactors (4000 m3 each) where the main feedstock was swine slurry together with complex mixture of agricultural plant and
animal wastes. Samples were diluted in water prior to CE analysis. The effect of sample storage in RT and -18C was studied.
Long chain fatty acids were analysed by GC-MSD. Bacterial profiles were analysed based on their DNA (16SrDNA).
Findings:
Acetic acid decreased fast when sample was stored in a room temperature. Correlation was observed between VFAs
and long chain FAs concentrations as well as bacterial profiles.
Conclusion & Significance:
CE was utilized successfully and is attractive method for the analysis of separate VFAs, NH4 and
nutrients in the biogas production site. Samples should be analysed in the sampling date if stored in RT or freezed for longer
storage time.
Biography
Jasmiina Haverinen, (Project researcher,
M.Sc. )in the Kajaani University Consortium, Unit of Measurement Technology (MITY), which operates under University
of Oulu, Finland. She has been working in the bio fermentation, food, and environmental sectors co-operating closely with companies.
Jasmiina.Haverinen@oulu.fiJasmiina Haverinen et al., J Bioremediat Biodegrad 2018, Volume 9
DOI: 10.4172/2155-6199-C1-015