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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Dhruti Mistry et al., J Biotechnol Biomater 2016, 6:6(Suppl)
Reduction in radiation inducedDNAdamage in human peripheral blood lymphocytes by treatments
of Alstonia scholaris bark extracts
Dhruti Mistry and Meonis Pithawala
Uka Tarsadia University, India
It is well established that radiation exposure causes DNA damage. Compounds within certain plants have often been targeted
as protectors against radiation induced DNA damage. In the present study, use of aqueous-methanolic extracts (50 µg per ml)
from bark of Alstonia scholaris as radioprotectors has been reported. The cytogenetic parameters studied as indices for DNA
damage were Chromosomal Aberrations (CAs) and Micronuclei (MN) frequency from human peripheral blood lymphocytes.
Blood was irradiated to 0, 2, 4 and 6 Gy of X-rays radiation (Source: 6MV X-ray Photons, Siemens, Oncor Expression Medical
Linear Accelerator). The irradiated samples were exposed to aqueous-methanolic bark extracts under three different situations:
To rule out whether presence of components in extract may not allow the damage to take place, blood samples were irradiated,
simultaneously exposed to extracts and then cultured; to rule out the possibility of possible DNA damage repair capability of
extracts, blood samples were irradiated and cultured for 24 hours and then exposed to extracts; and to rule out the possibility of
possible radioprotective properties of the extracts, blood samples were exposed to extracts first, cultured for 24 hours and then
exposed to radiation. In all three conditions, we found that presence of extracts had significant contribution in the reduction
of DNA damage, measured both as CAs as well as MN frequency. Since the study used crude extracts, number of compounds
present in the extracts might have played a role, some as protectors, some as mitigators and still certain with DNA damage
repair capabilities. Once the actual compounds present in the extracts be determined and their probable role be decided in
reducing radiation induced DNA damage, new sort of drugs be formulated with potential to protect against radiation damage.
Biography
Dhruti Mistry is currently pursuing her PhD in Applied Sciences (Biotechnology) from Uka Tarsadia University, India. She is also working as a Teaching Assistant
at C G Bhakta Institute of Biotechnology. She has 4 research papers and 6 review articles published in peer reviewed national and international journals. She is a
Member of the Society for Ethnopharmacology, Kolkata, India.
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