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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Zainab Zahid Saadoon et al., J Biotechnol Biomater 2016, 6:6(Suppl)
Tracing the neuroblasts from subventricular zone to olfactory bulb in adult mice brains using anti-
doublecortin antibodies and H&E stain
Zainab Zahid Saadoon and Huda Mahdi Al Khateeb
Baghdad University, Iraq
Background: Neural stem cells are not confined to the embryonic development of the brain as believed in the past; in contrasts,
it continues life-long in the adult mammalian brains even in non-pathological state. Neural stem cells and their progeny,
the neuroblasts are residents in the subventricular zone (SVZ) of the lateral ventricle in the adult mammalian brains. The
neuroblasts after they were produced in the SVZ would migrate in a well-defined pathway emerged from the SVZ and directed
towards the olfactory bulb (OB). This pathway is called the Rostral Migratory Stream (RMS) and its main components are the
neuroblasts.
Aims: To trace the chain arrangement of neuroblasts along the RMS using Hematoxylin and Eosin and the specific marker of
the neuroblasts "anti-doublecortin antibody”, describing grossly how the pathway emerges from SVZ proceeding rostrally to
the OB in the adult mice brains.
Materials &Methods: Adult mice brains from both brain sexes were used in this experiment to view both coronal and sagittal
sections upon which Hematoxylin and Eosin and immunohistochemical staining were exploited. Anti-doublecortin antibody,
the specific marker of the immature neurons was used in the immunohistochemical staining of this study.
Results: This study revealed clustering of the neuroblasts in the SVZ and while this special arrangement carried out at the
SVZ, the neuroblasts changed their arrangement when being traced sagittaly into chain-like strip of cells forming a sigmoidal
shape stream. The chain of the neuroblasts in the stream demonstrated changing in shape and direction throughout its length
with special arrangement at its starter from the lateral ventricle forming a funnel shape limb before joining the rest of stream.
It delineated four limbs here along its pathway.
Conclusion: The neuroblasts take different arrangement through their period of life from their site of origin to their final
destination, the olfactory bulb through the RMS. In the stream the neuroblasts follow a sigmoidal shape pathway described
here as four limbs instead of 3 ones in previous studies. The new described part is the funnel shape limb which is named the
infundibulum at which the neuroblasts in the stream starting up their migration from SVZ before they join the next limb, the
vertical limb.
Biography
Zainab Zahid Saadoon is an Assistant Lecturer at College of Medicine, Baghdad University, Iraq. She has completed her MSc and currently pursuing PhD at
College of Medicine, Baghdad University, Iraq.