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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Pranab Goswami, J Biotechnol Biomater 2016, 6:6(Suppl)
Development of DNA aptamers against human heart type fatty acid binding protein for early
detection of acute myocardial infarction
Pranab Goswami and Ankana Kakoti
Indian Institute of Technology Guwahati, India
C ardiovascular diseases are the single greatest cause of adult mortality globally, constituting about 31% of all global deaths.
Detection of cardiovascular diseases has thus emerged as not only a social and clinical issue but also as an economic one.
The current investigation is centered on the development of specific aptamers against human heart type fatty acid binding
protein (FABP3), a novel early marker for detection of acute myocardial infarction (AMI). It also encompasses the detection of
FABP3 using the developed aptamers on a specially designed paper based microfluidic device (µPAD). Two ssDNA aptamers,
N13 and N53 were isolated through Systematic Evolution of Ligands by Exponential Enrichment (SELEX) against human
heart-type fatty acid binding-protein (FABP3). The aptamers bound to FABP3 with dissociation constants 0.0743±0.0142 µM
and 0.3337±0.1485 µM, respectively. The aptamers displayed stable behavior at different pH, temperature and ionic strength.
Considering the large sizes of the aptamers, limited proteolysis of the aptamer-protein complex was performed to map the
amino acids involved in binding, which was then used to screen docked structures. The N13 led interaction with stronger
affinity, involving more salt bridges and fewer hydrogen bonds, whereas N53 had less number of salt bridges with higher
number of hydrogen and hydrophobic interactions. The greater footprint of N53 incited synergistic conformational changes in
N53 and FABP3 leading to decrease in binding affinity during the recognition. The aptamers so developed and characterized
were then used to detect FABP3 on a paper based microfluidic device designed for the same with leak proof property and low
cost. An aptamer modified gold nanoparticle aggregation assay was used as the Yes/No format for the detection of FABP3 with
a minimum detection limit of 54 ng per ml.
Biography
Pranab Goswami has completed his PhD degree from Gauhati University during 1994. From 1991 to 2002, he was a Scientist at CSIR, India. He was a BOYSCAST
Fellow of DST, India at University of Massachusetts, Boston. He has joined IIT Guwahati in 2002 and became Professor in 2009. He was the Founder Head of CIF
Centre during 2004 to 2006 and Head, Biotechnology Department during 2006 to 2009 at IIT Guwahati. Currently he is the Head of Energy Centre at IIT Guwahati.
He is working in the field of biosensors with focus on developing novel biorecognition system for various diagnostic applications.