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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Sahaya Sukeetha D et al., J Biotechnol Biomater 2016, 6:6(Suppl)
Exploration and Purification of Bioactive compound from seaweeds against human bacterial
pathogen
Sahaya Sukeetha D1 and John Antony B2
1Research Scholar, Bharathidasan University, Trichy, India
2MPEDA, Nagapattinam, India
Treatment of Infectious diseases by the use of commercially available drugs are becoming certain limitations due to changing
patterns of resistance in pathogens and causing side effects. These limitations demand for improved pharmacokinetic
properties which necessitates continued research for the search of new novel drugs. Marine organisms are rich source of
structurally novel and biologically active metabolites. The cell extracts and active constituents of various algae and seaweeds
have been shown to have antibacterial activity against gram positive and gram negative bacteria. Hence the crude extracts
from the seaweeds Amphiroa foliacea, Chactomorpho tortuosa, Caulerpa scalpelliformis and Sargassum sp were tested for
their resistance against multidrug resistance pathogens such as Staphylococcus aureus, Klebsella sp, Proteus sp. The extracts
were obtained with the solvents methanol, chloroform, ethyl acetate and hexane. A highest zone of inhibition was observed
in the hexane extract of Amphiroa foliacea and ethyl acetate extract of Sargassam sp against Proteus sp, Staphylococcus
aureus respectively. An inhibition zone of 8mm was observed in ethyl acetate extract of Sargassam sp against Staphylococcus
aureus. Further the extract of Sargassum sp was purified using silica column chromatography. Single compound fractions
were separated and each fraction was screened for antibacterial activity against Staphylococcus aureus. F4 fraction possessed
antibacterial activity of 7mm which is similar to crude extract. Further the F4 fraction is subjected NMR analysis. Ethyl acetate
fraction was found to be posses α-hydroxy stearic acid.
Biography
D. Sahaya Sukeetha ., M.Phil., (Ph.D) is an graduate from Bharathidasan University. Presently she is working as a scientific officer in the department of
molecular biology, Inbiotics, Nagercoil. She had worked as an Assistant professor for 7 years and handled biotechnology classes for both under graduate and post
graduate students. Also she had guided 12 project post graduate students. In her academic career she had published 5 research papers and attended various
conferences.