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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Hosam Gharib Abdelhady, J Biotechnol Biomater 2016, 6:6(Suppl)
4D molecular scale imaging of the effect of suicide anticancer nano-particles on individual live
cancer cells
Hosam Gharib Abdelhady
Taibah University, Saudi Arabia
The unique ability of the real time Atomic Force Microscopy (AFM) in providing valuable information in the field of
biomolecular sciences, gene delivery and cancer therapy will be demonstrated. For example, the formation of the most
durable, individual, DNA nanoparticles (DNPS) able to resist the enzymatic digestions of cancer cells and evoke apoptosis was
monitored in 4 dimensions (4D) from the first second of the interaction between individual DNA molecules and individual
dendrimers nano-polymers till the death of individual cancer cells in their environment. Optimizing both, the incubation
time between DNA/dendrimers and the DNA/dendrimers ratios have shown significant effects in producing the best DNPS.
Finally, cancer cells were exposed to the ideal DNPS and directly imaged by 4D AFM. Cell membrane liquefaction, cytoplasmic
shrinkage, cytoskeleton structure loss and changes in cellular nanomechanical properties were observed. In contrast, control
cells have no changes. Thus, understanding the real-time effects of anticancer DNPS on the cytoskeletal and nanomechanical
behaviors of cancer cells may provide new methods for cancer treatment.
Biography
Hosam Gharib Abdelhady has completed his PhD in Biophysics and Surface Analysis from College of Pharmacy, University of Nottingham in 2004 and Postdoctoral
studies with Professor Donald Dendrimers at Central Michigan University in 2005. He has then served as a Senior Scientist and directed the Analytical Department
at Dendritic Nanotechnologies, CMURC, Michigan from 2005-2009. He is currently an Associate Professor at College of Pharmacy, Taibah University, Saudi Arabia.
He has published more than 15 papers in reputed journals, a patent in Nanomedicine and received more than $ 1M grants.