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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
J Biotechnol Biomater 2016, 6:6(Suppl)
Bacteriophage-antibiotic synergism to control planktonic and biofilm producing clinical isolates of
Pseudomonas aeruginosa
Manal Mohammad Baddour
Alexandria University, Egypt
Introduction: Pseudomonas aeruginosa is a highly resistant opportunistic pathogen and is capable of forming biofilms on
medical devices. Bacterial biofilms, which are micro-colonies encased in extracellular polysaccharide material are so difficult
to be treated by conventional antibiotics. During the last decade, P. aeruginosa phages have been extensively examined as an
alternative to antimicrobial agents.
Aim: The aim of the study was to assess bacteriophage-antibiotic combination on planktonic and biofilm states of P. aeruginosa
isolates.
Materials: In this study, we isolated 6 lytic phages from hospital effluents; they were tested against 50 P. aeruginosa strains,
isolated from different clinical specimens delivered to the Diagnostic Microbiology Laboratories, Faculty of Medicine,
Alexandria University.
Results: Out of the 50 isolates, 15 were susceptible to these phages. So the biofilm forming capacity of these 15 isolates was
investigated. The results showed that 14 isolates (93.33%) produced detectable biofilm. The minimum inhibitory concentration
(MIC) and minimum biofilm eradication concentration (MBEC) assays were used to evaluate the antibiotic sensitivity patterns
of these P. aeruginosa isolates in their planktonic and biofilm phases to amikacin and meropenem. Also, the effects of phage on
the planktonic and biofilm states of isolates at different multiplicities of infections (MOI) were tested. On the planktonic state,
Amikacin-phage combination showed synergistic effect (P=0.001) and Meropenem-phage combination showed synergistic
effect (P=0.003). On the biofilm state, Amikacin-phage combination showed biofilm eradication in 50% of the isolates
(P=0.003). On the other hand, Meropenem-phage combination showed biofilm eradication in only 14.3% of the strains.
Conclusion: The combination of phage and antibiotics could have potentially more benefits on P. aeruginosa planktonic and
biofilm states than just using phages or antibiotics alone.
The art of mathematical biology
Mona A Mahran
Alexandria University, Egypt
Mathematical biology is a fast-growing, well-recognized subject and one of the most exciting modern applications of
mathematics. The increasing use of mathematics in biology is inevitable as biology becomes more quantitative. The
complexity of the biological sciences promoted interdisciplinary involvement. For the mathematician, biology opens up new
and exciting branches, while for the biologist, mathematical modeling offers another research tool commensurate with a new
powerful laboratory technique but only if used appropriately and its limitations recognized. Currently, it seems that theoretical/
mathematical biology offers lots of promising perspectives and possibilities for mathematicians and theoretically interested
biologists. Mathematical biology has been successfully applied in many fields as for example; cancer detection, antiretroviral
therapy including the integrase inhibitor Raltegravir in HIV-1 patients and molecular dynamics.