bio-summit-2016_posters-accepted-abstracts

11 / 16

Page 67
Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
J Biotechnol Biomater 2016, 6:6(Suppl)
Synthesis and application of novel tricyanofuran hydrazone dyes as sensors for detection of
microbes
Hatem Elsayed Mohamed Hussien Gaafar
National Research Centre, Egypt
Acknowledging the need todevelop rapid and sensitive bacterial recognition approaches, we functionalized the tricyanofuran
hydrazone molecular switch. Of significant interest in relation to the synthesized hydrazones is the formation of two
different conjugated structures upon exposure to different pH values. Many bacteria release ammonia gas, which alkalizes
environments. Herein we report the synthesis of a tricyanofuran hydrazone having the function of a colorimetric pH sensor.
The UV-visible absorption and fluorescence spectra exhibit reversible color changes of the tricyanofuran hydrazone solution in
acetonitrile under acid-base conditions. Our results indicate that the tricyanofuran hydrazone probe can identify the bacterial
targets quickly with high sensitivity. The infected samples exhibit a significant color change from orange to blue and in the
mean time there is a decrease in fluorescence emission as a function of ammonia and volatile amines released from bacterial
metabolites. This tricyanofuran hydrazone chromophore is proposed for use in food packaging with a pH-sensing capability.
Alpha cyano-4-hydroxy-3-methoxycinnamic acid inhibits proliferation and induces apoptosis in
human breast cancer cells
Lamia Hamdan Ramdani1, Zoheir Arrarz2, Yacoub Al Muataz2, Lutfi Suleiman2
1Centre de Recherche Scientifiqueet Technique en Analyses Physico-Chimiques, Algeria
2University of Auvergne, France
Breast cancer is the leading cause of cancer death in women worldwide and a critical public health concern. In this context,
the present work has examined the in vitro and in vivo antiproliferative and pro-apoptotic effects of α-cyano-4-hydroxy-
3-methoxycinnamic acid ACCA on human breast cancer cell lines, MDA-MB-231, MCF-7 and T47D. Treatment with ACCA
resulted in dose and time-dependent decrease of cell proliferation, viability in colony formation assay and in induction of
programmed cell death (apoptosis) with minimal effects on non-tumoral cells. The ability of ACCA to suppress growth in
cancer cells not expressing or containing defects in p53 gene indicates a lack of involvement of this critical tumor suppressor
element in mediating ACCA-induced growth inhibition. The stimulation of breast cancer cells with ACCA would increase the
expression of the ratio of Bax to Bcl-2, a process widely involved in the stimulation of apoptosis. Besides, we have demonstrated
the ability of ACCA to inhibit the migration and invasion of MDA-MB231 cells. Additionally, tumor growth of MDA-MB-231
breast cancer cells was dramatically affected in vivo by ACCA.Therefore, these results show that ACCAmight be very promising
therapeutic targets in the treatment of breast cancer.