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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
J Biotechnol Biomater 2016, 6:6(Suppl)
Amyloid-like protein membrane: A natural polymer based biosensing material
Gozde Kabay
TOBB University of Economics and Technology, Turkey
Electrospinning has been a popular technique to obtain nanofibrous membranes from synthetic and natural polymer sources.
In contrary to synthetic polymers, the production capabilities of natural polymermembranes failed at some extend bymeans
of electrospinnability and efficiency also biocompatibility. In this paper, we introduced an enzyme immobilization platform
from a natural polymer membrane. For this purpose, a model protein bovine serum albumin (BSA) was chosen mainly for
enhanced supporting property. To procure electrospinnable solution of BSA, beta-mercaptoethanol (β-ME) was used to induce
tertiary structure and low ratio (1.5:1.0 TFE:PBS (pH: 7.4)) of 2,2,2-triflouroethanol (TFE) was added as a stabilizing agent,
respectively. The electrospun membranes were activated with RF plasma treatment by employing ethylenediamine (EDA) as a
precursor to incorporate amino (-NH2) groups on the surface. Those surfaces were cross-linked with glutaraldehyde aqueous
solutions at concentrations between 0.01 and 5% wt. which followed by the covalent attachment of glucose oxidase (GOD).
The performance of enzyme immobilized membranes was tested by employing amperometric measurements against various
glucose concentrations in terms of response time, enzymatic activity and linearity. The effects of plasma parameters and cross-
linking conditions on the performance of protein membrane based enzyme electrode were also studied.
Phenotypic and genotypic characterization of Staphylococcus aureus strains from some food stuff
of animal origin
Belgin Siriken1, T Yildirim2, A K Guney1, I Erol3 and B Durupinar1
1Ondokuz Mayis University, Turkey
2Amasya University, Turkey
3Republic of Turkey Ministry of Food, Agriculture and Livestock, Turkey
Methicillin resistant Staphylococcus aureus (MRSA) is one of the most important public health problems in many
countries. In recent years, the existence of MRSA in foodstuff of animal origin and its transfer among farm animals,
foodstuff of animal origin and human beings have been shown with molecular typing studies. The objective of this study was
to investigate existence, methicillin resistance (MR) and clonal relationship of Staphylococcus aureus (S. aureus ) strains from
foodstuff of animal origin consumed in the Samsun region of Turkey. In this study, a total 175 coagulase positive staphylococci
(CPS) strains were isolated from meat (n=110), milk (n=56) and fishery products (n=9). From these, 62 S. aureus strains were
identified from meat (n=44), milk (n=9) and fishery products (n=9). Identification and MR properties of the isolates were
confirmed by PCR technique in which appropriate primers for nuc and mecA gene were used. For detection of MR, we also
used minimal inhibitory concentration (MIC) technique. We compared two techniques; although 21 isolates were determined
as MRCPS using MIC (≥12 µg), 18 isolates were detected MRCPS using PCR assay. Among these, 15 isolates were identified as
MRSA using PCR technique. We investigated only MRSA isolates for the clonal relationship using PFGE method. PFGE typing
of the 15 MRSA strains yielded 6 PFGE patterns. Pattern A and E were found to be dominant types in our study. Pattern E
consisting of 7 strains was from fishery products. Pattern A consisting of 4 strains was frommeat and fishery products. Patterns
B, C, D and F were single isolates from milk, meat and milk products, respectively.
,