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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Sahaya Sukeetha D et al., J Biotechnol Biomater 2016, 6:6(Suppl)
Exploration and Purification of Bioactive compound from seaweeds against human bacterial
pathogen
Sahaya Sukeetha D1 and John Antony B2
1Research Scholar, Bharathidasan University, Trichy
2MPEDA, Nagapattinam
Treatment of Infectious diseases by the use of commercially available drugs are becoming certain limitations due to changing
patterns of resistance in pathogens and causing side effects. These limitations demand for improved pharmacokinetic
properties which necessitates continued research for the search of new novel drugs. Marine organisms are rich source of
structurally novel and biologically activemetabolites.The cell extracts and active constituents of various algae and seaweeds have
been shown to have antibacterial activity against gram positive and gram negative bacteria. Hence the crude extracts from the
seaweeds Amphiroa foliacea, Chactomorpho tortuosa, Caulerpa scalpelliformis and Sargassum sp were tested for their resistance
against multidrug resistance pathogens such as Staphylococcus aureus, Klebsella sp, Proteus sp. The extracts were obtained
with the solvents methanol, chloroform, ethyl acetate and hexane. A highest zone of inhibition was observed in the hexane
extract of Amphiroa foliacea and ethyl acetate extract of Sargassam sp against Proteus sp, Staphylococcus aureus respectively.
An inhibition zone of 8mm was observed in ethyl acetate extract of Sargassam sp against Staphylococcus aureus. Further the
extract of Sargassum sp was purified using silica column chromatography. Single compound fractions were separated and each
fraction was screened for antibacterial activity against Staphylococcus aureus. F4 fraction possessed antibacterial activity of
7mm which is similar to crude extract. Further the F4 fraction is subjected NMR analysis. Ethyl acetate fraction was found to
be possess α-hydroxy stearic acid.