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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
Study heterotrophic growth of Chlorella spp. under different carbon-to-nitrogen and carbon-to-phosphorous
ratios
Catalina Andrea Lugo De Ossa
1
, Mariana Peñuela Vásquez
2
, Natalia Andrea Gómez Vanegas
3
, Juan Martin Delgado
4
1Industrial and Environmental Microbiologist, 2. PhD. in Technology of Chemical and Biochemical Processes, 3. PhD. in Engineering, 4. Msc. In Engineering.
Bioprocess Research Group, Universidad de Antioquia, Medellín, Colombia
M
icroalgae have caused interest in recent years because of their particular way of accumulating lipids. These microorganisms can
be cultivated in an autotrophic, mixotrophic, and/or heterotrophic way. Heterotrophic cultures decrease growth time, increase
biomass concentration, and total lipid yields. Appropriate composition of the culture medium will favor the growth of the cells, for
this reason commercial culture media have been modified to establish the effect of increasing or decreasing the concentration of some
nutrients when producing biomass and lipids. However, to achieve good lipid yields, it is necessary to ensure a high concentration
of biomass at an initial stage of cultivation. Lipids, being primary metabolites, can be induced by subjecting the microalgae to
stress conditions depending on both the species and the abiotic factors. This work evaluated the heterotrophic growth of the native
microalga
Chlorella
sp using glucose as carbon source and varying relations carbon/nitrogen and carbon/phosphorus to favor the
production of biomass. In addition, the change of fatty acid composition changes with biomass production. Maximum biomass
obtained was 9.25g/L and 8.67g/L for C/N of 25:1 and C/P of 200:1 during 7 days of cultivation, their productivities were 0.93g/L*d
and 0.99g/L*d. Total fatty acid production was favored with C/N 50:1 and C/P 400:1 reaching 25.7% and 22% of total fatty acids in dry
biomass, also higher fatty acid productivities in biomass of 41.16mg/L*d and 24.32mg/L*d with C/N 10:1 and C/P 200:1. Low C/N
and C/P ratios stimulated biomass production, biomass lipid productivity, and decreased total fatty acid production. High C/N and
C/P ratios improved the production of total fatty acids. In this way, the maximum production of biomass must be reached for further
achieving the stage of nutritional exhaustion due to the deficiency of N and P in the culture medium. This causes the elongation of
polyunsaturated fatty acid chains.
Biography
Catalina Andrea Lugo De Ossa is an Industrial and Environmental Microbiologist from Universidad de Antioquia, Medellin, Colombia, She belongs to Bioprocess Research
Group in the microalgae investigation line. She participated in the design of culture medium for the induction and production of intracellular fatty acids, proteins, secondary
metabolites and water bioremediation of heavy metals using wild microalgae isolated from water sources in Colombia.
catalina.lugo@udea.edu.comCatalina Andrea Lugo De Ossa et al., J Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-080