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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
Production of bioactive pigments in cell lines of quinoa (
Chenopodium quinoa
)
Josefa Escribano-Cebrián
1
, Paula Henarejos-Escudero
1
, Berenice Guadarrama-Flores
1
, M Alejandra Guerrero-Rubio
1
, Luz Rayda Gómez-Pando
2
, Francisco
García-Carmona
1
and
Fernando Gandía-Herrero
1
1
Universidad de Murcia, Spain
2
National Agricultural University La Molina, Peru
B
etalains are water-soluble, nitrogen containing pigments present inmost plants of the order Caryophyllales. Betalains are classified
into two structural groups: betacyanins (violet) and betaxanthins (yellow). Both groups share betalamic acid as the structural and
chromophoric unit. It is condensed with amines and amino acids in betaxanthins and with
cyclo
-DOPA in betacyanins. Betalains are
bioactive molecules with high antioxidant and free radical scavenging activities and a strong chemopreventive potential. The present
research was focused on the establishment of callus cell lines derived from the plant quinoa (
Chenopodium quinoa
). Quinoa belongs to
the family Amaranthaceae and thus to the betalain producing order Caryophyllales. Cell lines were developed on semisolid medium
and the betalamic pigments synthetized by different lines have been identified by HPLC-DAD-MS/MS, as well as the pigment content
present in the seedlings used as explants. Violet callus cell lines of
Chenopodium quinoa
were developed from hypocotyls of differently
colored Peruvian quinoa grains varieties (Figure C), recently described as source of betalains. In callus, the major pigment identified
was betanin, while in seedlings one of the major pigments detected was the non-glycosylated and hydroxylated precursor of the
violet compounds, betanidin. For the first time betalain producing callus lines derived from quinoa were established. This offers an
opportunity to develop cell suspension cultures to be used as bio-factories in the production of bioactive pigments betalains. The
controlled production of theses pigments to be used as functional natural colorants may be of interest for the food, pharmaceutical
and cosmetic industries.
Biography
Josefa Escribano-Cebrián was trained as a Biochemist at the Department of Biochemistry and Molecular Biology of the University of Murcia (Spain). She got her PhD in
1986 and since then she has been working in Plant Biochemistry. She has publications in national and international journals. Currently, her research project combines
different approaches and multiple techniques to study the functional capacity of a family of bioactive plant compounds-the betalains
pepa@um.esJosefa Escribano-Cebrián et al., J Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-080