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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
Effect of elicitors on the antioxidant activity of different plant cell cultures
Lorena Almagro, Carlos Cerón, Ana Belén Sabater-Jara, Begoña Miras-Moreno, Pedro Joaquín Sánchez-Pujante, María Borja-Martínez, María Isabel González-
Sánchez, Edelmira Valero-Ruiz, María Angeles Pedreño
Universidad de Murcia, Spain
Statement of the Problem:
Grapevine is a good source of antioxidant compounds like phenolic compounds which are important
in human nutrition. Likewise, broccoli contains a wide range of nutrients, dietary fibre, and phytochemicals with health-related
properties. On the other hand, safflower is a medicinal plant, which has red and yellow pigments that can be used as food colorants
or in the cosmetic industry. Safflower also produces bioactive compounds such as tocopherols and carthamin. Due to the high
value of these compounds, new strategies have been used in order to increase their production since their extraction from raw plant
materials is often tedious, expensive and the extracts are often heterogeneous. In this way, we have developed a method of production
of antioxidant compounds based on the elicitation of plant cell cultures with β-cyclodextrins (CD) separately or in combination with
other elicitors such as methyl jasmonate, coronatine, NaCl, β-glucan and hexenol.
Methodology:
We have evaluated the effect of CD, methyl jasmonate, coronatine, NaCl, glucan and hexenol on the antioxidant
activity in extracellular medium of grapevine, broccoli and safflower cell cultures for 144 h of incubation.
Findings:
In this work, we have observed that the highest levels of antioxidant activity were found in safflower cell cultures elicited
with β-glucan, β-glucan+CD or hexenol+CD. In addition, we also detected high levels of antioxidant activity in grapevine cell
cultures treated with CD and methyl jasmonate while no significant differences were observed in any of the treatments performed in
broccoli cell cultures compared to control cells.
Conclusion:
Grapevine and safflower cell cultures elicited were able to produce high levels of antioxidant compounds, and therefore,
these elicited plant cell cultures can provide an alternative system, which is at the same time, a more sustainable, economical and
ecological system for their production.
Biography
Lorena Almagro has completed her PhD from Murcia University and her Post-doctoral studies at the Institute of Molecular and Cell Biology in Porto (Portugal). In 2014,
she had a Post-doctoral position in the University of Murcia. She has received her first award for Applied Research in a private Company by CEEIM (2009) and European
Doctorate Awards (2013). She has published 25 papers in reputed journals and her work has been focused on the production and identification of bioactive compounds
derived from different plant cell cultures under elicitation.
lorena.almagro@um.esLorena Almagro et al., J Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-080