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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
Semi-synthesis of lysine-betaxanthin and its fluorescent properties
Cabanes Cos J, Gandía-Herrero F, Jiménez-Atiénzar M, García-Carmona F
and
Escribano-Cebrián J
Universidad de Murcia, Spain
B
etalains are nitrogen-containing natural pigments that provide bright coloration to fruits, flowers, and roots of plants of the
Caryophyllales order and present autofluorescence after excitation with blue light. They are divided into two groups: violet
betacyanins, with absorbance spectra centered at wavelengths around λm= 536 nm, and yellow betaxanthins, with absorbance spectra
centered at wavelengths around λm = 480 nm. Both groups share betalamic acid as their structural and chromophoric unit, which is
condensed with
cyclo
-DOPA in the betacyanins and with amines and amino acids in the betaxanthins In this work, the semi-synthesis
of lysine-betaxanthin from betanin, purified from red beet juice concentrate has been carried out. Basic hydrolysis of betanin released
betalamic acid, whose aldehyde group was condensed with the amine group of lysine. Immediately after synthesis, lysine-betaxanthin
was partially purified by solid phase extraction with a C-18 column. The pigment was characterized by absorbance spectroscopy and
HPLC-DAD analysis. Electrospray ionization mass spectrometry analysis (HPLC-ESI-MS-MS) was applied to elucidate the pigment
nature. Since lysine has two amine groups, one α and one ε, the
in-vitro
reaction of the amino acid with betalamic resulted in the
formation of two adducts. In this work, the native fluorescence of lysine-betaxanthin has also been characterized, by using an aqueous
solution of the pigment for registration of the fluorescence spectrum.
Biography
Cabanes Cos J, trained as a Biochemist at the Department of Biochemistry and Molecular Biology A of the University of Murcia (Spain). She got her PhD in 1986 and since
then she has been working in Plant Biochemistry and Biotechnology. She has been teaching for more than 30 years Biology and Biotechnology both in University of Murcia.
She also teaches Master’s of Molecular Biology and Biotechnology of the University of Murcia. Currently, her research project combines different approaches and multiple
techniques to study the functional capacity of a family of bioactive plant compounds -the betalains
jcabanes@um.esCabanes Cos J et al., J Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-080