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.com
Volume 7, Issue 4 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
Bio America 2017
October 19-20, 2017
October 19-20, 2017 | New York, USA
18
th
Biotechnology Congress
UHPLC-MS/MS analysis of extracts of
Sinningia magnifica
(Gesneriaceae) from seedlings cultivable
in-
vitro
, callus and
in-nature
Alessandra F Serain, Viktor K Nunes, Maria E A Stefanello
and
Marcos J Salvador
University of Campinas, Brazil
T
he
Sinningia magnifica
belong to Gesneriaceae family which comprises of around 150 genera and 3500 species, distributed in
tropics and subtropics around the world. In Brazil, phytochemical studies with
Sinningia
reported the identification of phenolic
glycosides, anthocyanins and anthraquinones with biological properties. The
in-vitro
micropropagation is a way of maintaining
healthy and uncontaminated explants for tissue culture application. The aim is to use the explants of S. magnifica to target callus and
cell culture in suspension, which has been an excellent alternative for the exploration of secondary metabolites. In this work, initially
a comparison was made between in-natura plant,
in-vitro
cultured seedlings and callus, to detect differences and similarities by
UHPLC-MS and parity with some compounds of our research group by UHPLC-MS/MS. Thus, fruits of
S. magnifica
were collected
and opened to release the seeds in Murashige e Skoog, 1962 (MS) medium, and cultivated in a photoperiod of 16/8 light/dark,
26±1ºC, with the leaves and the formation of calogenesis was induced with the use of growth hormones. Then, tubers and leaves of
S.
magnifica
in-nature
and
in-vitro
, and callus, which was dried at 40°C was collected, and subjected to the maceration with ethanol and
determined the chemical composition by UHPLC-MS, and which was used in isolated compounds for comparison by UHPLC-MS/
MS. The comparison showed common compounds and differences between the
in-natura
plant and the plant that grew in a controlled
environment, as well as the callus, reinforced by the UHPLC-MS/MS analysis. Despite the positive results, further investigations
are necessary to confirm the potential of this technique for the production of bioactive compounds and the real potential of the
compounds for biological application.
Biography
Alessandra F Serain is currently a Pharmacist from the State University of Campinas and a PhD candidate with FAPESP scholarship, in the Biosciences and Technology
Program of Bioactive Products of the DBV / IB - UNICAMP with the research project that seeks to perform a phytochemical study of two species of
Sinningia
for application
in Photodynamic Therapy (PDT) in culture of human cells, and biotechnological study of plant cell culture. She has her experience of Scientific Initiation at BTPB-UNICAMP,
18 months with FAPESP scholarship, in the field of phytochemistry, PDT, essential oils and microbiological activity
ale.serain@gmail.comAlessandra F Serain et al., J Biotechnol Biomater 2017, 7:4 (Suppl)
DOI: 10.4172/2155-952X-C1-080