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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 33
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Analysis of olfactory genes in Sitobion avenae by antennal transcriptome
Julian Chen, Wenxin Xue, Jia Fan and Jingrui Sun
Chinese Academy of Agricultural Sciences, China
Olfaction is the essential sense of insect’s feeding and mating. It achieves semiochemical and pheromone perception through
olfactory pathway which coupled components by expressing series olfactory genes, distributing in antenna. transcriptome
of antenna is a good way to understand the complicated olfactory system components. Analysis of chemical sensing system is a
crucial basic work for studying the olfactory physiology of aphids and developing biological control technologies. In this study,
we sequenced winged and wingless antennae transcriptome of Sitobion avenae (Fabricius), one of the most serious pests of cereals
using IlluminaHiSeqTM2500/MiseqTM technology and Trinity assembly. A total of 133,331 unigenes were obtained with an average
length of 594 bp. To annotate the transcripts, we searched against the Nr, Nt, Pfam, KOG/COG, Swiss-prot, KEGG and GO databases
and 100,345 unigenes (75.26%) were annotated in at least one database. There were 1,517genes (1,107 up-regulated and 410 down-
regulated genes) differentially expressed between wingless and winged antennae, respectively. Nine of them are associated with odour
binding pathway. 13 OBPs, 5 CSPs genes were identified from S. avenae. All of these transcripts have the typical structural features
of insect (six conserved cysteines for OBPs and 4 for CSPs). Through multiple comparisons phylogenetic tree constructed of OBPs
across several aphids, we can see that there is a high similarity between orthologs within a range of aphid species. Besides, 48 Ors
(odorant receptors) were annotated as well and further studies are in progress.
Biography
Julian Chen has completed her PhD at Graduate School & Institute of Plant Protection, Chinese Academy of Agricultural Sciences (IPP-CAAS). She is a Group
Leader of Wheat Insect Pest Research Group, IPP-CAAS. She has published more than 110 papers in reputed peer-review journals.
Julian Chen et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)