Plant-Genomics-2016_Scientific-Tracks-Workshop

3 / 60

Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 29
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
The nitrate inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases
wheat yield
Xue He, Wenjing Li, Baoyuan Qu, Xueqiang Zhao, Wenying Ma, Bin Li, Zhensheng Li and Yiping Tong
Institute of Genetics and Developmental Biology-CAS, China
Nitrate is a major nitrogen resource for cereal crops, thus understanding nitrate signaling in cereal crops is valuable for engineering
crops with improved nitrogen use efficiency. Although several regulators have been identified in nitrate sensing and signaling
in Arabidopsis, the equivalent information in cereals is missing. Here, we isolated a nitrate inducible and cereal specific NAC
transcription factor TaNAC2-5A from wheat (Triticum aestivum). CHIP-SEQ (Chromatin Immunoprecipitation based Sequencing)
dates indicated that TaNAC2-5A could bind to the genes encoding nitrate transporter and glutamine synthetase and the genes
involving auxin signaling pathway. And a RING Zinc-finger protein that was screened by yeast two-hybrid may regulate the response
of TaNAC2-5A to nitrate. Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate and hence increase
root ability to acquire nitrogen. Further, we found that TaNAC2-5A over-expressing transgenic wheat lines had higher grain yield
and higher nitrogen accumulation in aerial parts and allocated more nitrogen in grains in a field experiment. These results suggest
that TaNAC2-5A is involved in nitrate signaling and show that it is an exciting gene resource for breeding crops with more efficient
use of fertilizer.
Biography
Xue He has completed her PhD from China Agriculture University in 2007 and joined the Tong Fellow at Center for Molecular Agrobiology, Institute of Genetics and
Developmental Biology, Chinese Academy of Sciences. Her interest focuses on the genetic improvement of nitrogen use efficiency of wheat. She has published
research results in Plant Journal, Plant Physiology, New Phytologists, etc.
Xue He et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)