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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 35
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Induced defense responses in hop-Verticillium interactions as revealed by proteomic and
transcriptomic analysis
Branka Javornik1, Vasja Progar1, Stanislav Mandelc1, Jernej Jakse1, Natasa Stajner1, Andreja Cerenak 2, Sebastjan Radisek2 and Sabina Berne1
1University of Ljubljana, Slovenia
2Slovenian Institute for Hop Research and Brewing, Slovenia
Verticillium wilt caused by soil borne vascular tissue infecting species of the Verticillium genus is a devastating disease in
many crops. Host resistance and phytosanitary measures are the best options for controlling the disease so elucidation
of plant wilt resistance can undoubtedly enhance resistance breeding. We study Verticillium wilt in hop caused by a highly
virulent strain of V. nonalfalfae, which has recently become a serious threat to hop production in Europe. The first QTL for
hop resistance against Verticillium wilt has been identified, opening up possibilities for investigation of the genetic basis of
hop resistance source, as well as markers development and their exploitation in MAS resistant breeding. Transcriptomics and
proteomics were employed in a time course experiment comparing infected and control plants of resistant and susceptible hop
cultivars to characterize hop-Verticillium interactions. The expression patterns of most studied genes and the decline of fungal
biomass in the infected resistant cultivar suggest that the defense response in the resistant cultivar is strong enough at 20 dpi
to restrict further fungus colonization. The results so far have shown strong expression of genes encoding PR proteins in both
interactions, strong up regulation of genes implicated in ubiquitination and vesicle trafficking in the incompatible interaction
and their down regulation in susceptible plants. Additionally, the identification and high abundance of two mannose/glucose
specific lectin isoforms and dehydrins present only in the roots of the resistant cultivar implies a putative function of these
proteins in hop resistance against V. nonalfalfae.
Biography
Branka Javornik is working as Head of Biotechnology Studies in Biotechnical Faculty at University of Ljubljana, Slovenia. Her international experience includes
various programs, contributions and participation in different countries for diverse fields of study and interests reflect in wide range of publications in various national
and international journals.
Branka Javornik et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)