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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 30
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Molecular regulation of nitrate in plants
Yong Wang
Shandong Agricultural University, China
We identified a novel gene named Nitrate Regulatory Gene 2 (NRG2) by using forward genetics, which mediates nitrate signaling
in Arabidopsis.NRG2 mutants showed inhibited induction of nitrate responsive genes after nitrate treatment by an ammonium
independent mechanism. The nitrate content in roots was significantly lower in the mutants than in WT, which may have resulted
from reduced expression of NRT1.1 and up-regulation of NRT1.8. Genetic and molecular data suggest that NRG2 functions upstream
of NRT1.1 in nitrate signaling. Furthermore, NRG2 directly interacts with NLP7 in the nucleus but does not affect the nuclear
retention of NLP7 in the presence of nitrate. Transcriptomic analysis revealed that genes involved in four nitrogen related clusters
were differentially expressed in the NRG2 mutants. A nitrogen compound transport cluster was regulated by both NRG2 and NRT1.1,
while no nitrogen related clusters showed regulation by both NRG2 and NLP7. Thus, NRG2 plays a key role in nitrate regulation in
part through modulating NRT1.1 expression and may function with NLP7 via their physical interaction. NRG2 family consists of 16
members and each protein contains two uncharacterized functional domains: DUF630 and DUF632. We further investigated the role
of NRG2.10 and NRG2.15 in regulating nitrate signaling in Arabidopsis. The results showed that the induction of nitrate responsive
genes after nitrate treatments and the nitrate accumulation in seedlings were affected in both NRG2.10 and NRG2.15 mutants. These
findings demonstrate that NRG2 family members play important roles in nitrate signaling.
Biography
Yong Wang has obtained his PhD degree from University of Lausanne in Switzerland in 2006 and Postdoctoral studies from the University of California, San Diego
in USA. He has been working as a Professor at Shandong Agricultural University in China since 2010. He has published many papers in reputed journals including
The Plant Cell and Plant Physiology etc.
Yong Wang, Adv Crop Sci Tech 2016, 4:3 (Suppl)