Plant-Genomics-2016_Scientific-Tracks-Workshop

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Volume 4, Issue 3 (Suppl) Adv Crop Sci Tech
ISSN: 2329-8863 ACST, an open access journal
Page 39
Notes:
Plant Genomics 2016
July 14-15, 2016
conferenceseries.com
July 14-15, 2016 Brisbane, Australia
4th International Conference on Plant Genomics
Genome-wide association for pollen viability and silk receptivity under heat stress in tropical maize
Ayyanagouda Patil
University of Agricultural Sciences, India
Heat stress is a one of the major abiotic stresses which has profound impact on pollen viability and silk receptivity intern affect
maize yields. Heat stable lines can be identified based on pollen viability and silk receptivity under high temperature condition.
The present study is first of its kind to conduct genome wide association study for identification of genomic loci associated with
pollen viability and silk receptivity under heat stress in tropical maize using genotyping by sequence data (GBS) with 239,594 SNP
markers (MAF≥0.05) used for marker trait association in a panel of maize inbred lines. The pollen viability and silk receptivity of
the inbred lines was assessed based on seed set percent. Association analysis was conducted using a mixed linear model involving
both population structure and kinship to control false positives. The average physical distance between pairs of markers was 27.7
kb with mean LD estimation (r2) of 0.36 across genome and LD decay of 6.34 kb at r2=0.2. Out of 239,594 SNPs, 44 SNPs were
significantly (P≤0.0001) associated with pollen viability and 69 SNPs were significantly associated with silk receptivity under heat
stress. Candidate gene based analysis was used to predict the putative function of the associated genes. Of the many SNP makers, the
gene associated with SNP marker (S6_156252525) is homologue of rice Osg1 gene which code for β-1,3-glucanases associated with
pollen fertility. The SNP (S10_120824169) for silk receptivity was associated with protein phosphatase 2C, which has an important
role in phosphorylation/dephosphorylation of heat shock proteins, possibly promoting the silk to survive under high temperatures.
The SNP marker S3_220855063 was found to be associated with hydroxyproline-rich glycoproteins, which plays an important role
pollen tube and silk growth. These SNPmarkers linked to the functionality of silk and pollen may be the ideal candidate for developing
heat tolerant hybrids.
Biography
Ayyanagouda Patil has completed his Masters in Genetics and Plant Breeding from University of Agricultural Sciences, India and later pursued his Doctoral degree
in the Department of Plant Biotechnology. He has worked extensively on genomics & transcriptomics plant improvement for his doctoral studies. He has later joined
the University of Agricultural Sciences, Raichur as an Assistant Professor of Biotechnology in the year 2011. He was nominated as Head of the Department of
Molecular Biology and Agricultural Biotechnology in University of Agricultural Sciences, Raichur on 2014 and continuing till date. He has published more than 25
research papers and abstracts.
Ayyanagouda Patil, Adv Crop Sci Tech 2016, 4:3 (Suppl)