livestock-nutrition-2016_posters-accepted-abstracts

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Volume 4, Issue 2 J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal
Page 60
Livestock Nutrition 2016
July 21-22, 2016
conferenceseries.com
July 21-22, 2016 Brisbane, Australia
2nd International Conference on
Livestock Nutrition
Effects of dietary threonine levels on laying performance, offspring traits and its regulation of embryo
expressions of pTOR and TDH in Chinese yellow-feathered broiler breeder hens
Shouqun Jiang1, Zongyong Gou2, Long Li 2, Xiajing Lin3, Fang Chen3, Chuntian Zheng 4, Fayuan Ding5 and Zongyong Jiang5
1Guangdong Academy of Agricultural Sciences, China
2Guangdong Key Laboratory of Animal Breeding and Nutrition
3Guangdong Public Laboratory of Animal Breeding and Nutrition
4Key Laboratory of Animal Nutrition and Feed Science in South China
5Ministry of Agriculture, China
The effects of dietary threonine (Thr) level on performance, offspring traits and embryo protein deposition in broiler breeder
hens was investigated. A total of 720 Lingnan yellow-feathered broiler breeder hens were randomly divided into 1 of 6 dietary
treatments with 6 replicates per treatment (20 birds per replicate). The hens were fed either the basal diet (Thr 0.38%) or the basal diet
supplemented with 0.00%, 0.12%, 0.24%, 0.36%, 0.48% and 0.60%Thr from 29 w to 38 w. The results showed that Thr supplementation
produced quadratic positive responses in laying rate. Hatchability was higher in breeders fed 0.12% and 0.24% Thr than those of
control birds (P<0.05). Dietary supplemental Thr had significant effects on expressions of mucin 2 (MUC2) in duodenum, colon
and uterus and ZO-1 in duodenum of hens (P<0.05). In chick embryo at embryonic age 18, there were significant up-regulations
of dietary Thr levels on the transcripts of liver and breast muscle poultry target of Rapamycin, thigh threonine dehydrogenase,
duodenum and ileum amino-peptidase (P<0.05), but no effects on MUC2 expression of duodenum and ileum (P>0.05). Chick
livability and serum uric acid nitrogen concentration were increased and liver glutamic-pyruvic transaminase activity was decreased
by dietary Thr supplementation (P<0.05). It concluded that there were positive effects of adding Thr on laying production of breeder
hens and offspring performance and this was associated with the regulations of gene expressions related to amino acid transportation
and protein deposition. The optimal dietary Thr supplemental level was 0.298% or 0.388 g/d for broiler breeders.
The effect of in vitro rumen digestion on polyphenol content and free radical scavenging activity of apple
pomace
Vanja Djukic
University of Guelph, Canada
This project is part of a larger task pursuing the development of naturally produced, health-contributing, polyphenol-enriched milk
products that are derived from byproduct plant matter from agricultural sources. Apple pomace (AP), an abundant agricultural
byproduct in Canada has high polyophenol content (1000-1415 g Gallic acid equivalent/100 g dry weight). The objective of this
study is to evaluate the effects of in vitro rumen digestion on the total polyphenol content and free radical scavenging activity of AP.
AP will be collected fresh and kept at -20 °C until analysis. Rumen inoculum will be collected from a mature lactating cow and in
vitro digestion will be performed for 24 and 48 hours of digestion in an ANKOM RF Gas Production System as per manufacturer’s
instructions. After digestion, the samples will be centrifuged at 26.940 g and filtered through 0.2 um polyethersulfone filters to
remove bacterial cells. All samples (treated and untreated) will be freeze-dried and ground, after which polyphenols will be extracted
by solvent extraction (80% methanol at 21 °C for 1 hour). Fast Blue BB assay for total polyphenol quantification and 2,2-diphenyl-1-
picrylhydrazyl (DPPH) free radical scavenging activity assay will be performed on all samples in triplicate with 3 technical replicates
to assess the extent of polyphenol degradation in the rumen. The fraction of polyphenols remaining after in vitro digestion will be
roughly representative of the amount available for absorption into the cow’s bloodstream and subsequently the mammary gland.
J Fisheries Livest Prod 2016, 4:2 (Suppl)